HIV-1 gp120 up-regulation of the mu opioid receptor in TPA-differentiated HL-60 cells.

HIV-1 gp120 up-regulation of the mu opioid receptor in TPA-differentiated HL-60 cells. Research Abstract Details 

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HIV-1 gp120 up-regulation of the mu opioid receptor in TPA-differentiated HL-60 cells. Abstract Text:

Jose A Beltran,Anitha Pallur,Sulie L Chang,

Opioid abuse has been shown to exacerbate the immunosuppressive effects and pathogenesis of HIV infection. The mu opioid receptor (MOR) is present on immune cells, such as macrophages, and mediates the direct immunomodulatory effects of opioids. Through its surface glycoprotein, gp120, HIV-1 binds to surface receptors on target cells, including macrophages, to exert its pathological effects. Binding of gp120 to macrophages stimulates the cells to release various pro-inflammatory cytokines, including TNF-alpha, which has been shown to regulate transcription of the MOR gene. In this study, we examined the effects of HIV-1 gp120 on MOR expression in HL-60 human promyelocytic leukemia cells differentiated into macrophage-like cells by TPA. Using real time RT-PCR, we found that exposure to gp120 up-regulated MOR expression in TPA-differentiated HL-60 cells at the transcriptional level. The functionality of the gp120-induced MOR in these cells was confirmed based on morphine's inhibition of forskolin-induced intracellular cAMP, which was naloxone reversible. Exposure to gp120 also stimulated the release of TNF-alpha from TPA-differentiated HL-60 cells. Treatment with TNF-alpha neutralizing antibody, as well as blockage of TNF-alpha's actions by anti-TNF-alpha receptor type II (TNFR-II) antibody, inhibited gp120-induced up-regulation of MOR mRNA. Our data suggest that one of the mechanisms by which HIV-1 gp120 up-regulates the MOR in TPA-differentiated HL-60 cells is through autocrine/paracrine actions of TNF-alpha via the TNFR-II receptor.

HIV-1 gp120 up-regulation of the mu opioid receptor in TPA-differentiated HL-60 cells. Publishing Authors By Initials

JA Beltran,A Pallur,SL Chang,

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HIV-1 gp120 up-regulation of the mu opioid receptor in TPA-differentiated HL-60 cells. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: International immunopharmacology

VOLUME: 6

Page Numbers: 1459-67

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ISSN: 1567-5769

DAY: 30

MONTH: 05

YEAR: 2006

HIV-1 gp120 up-regulation of the mu opioid receptor in TPA-differentiated HL-60 cells. Information

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LANGUAGE: eng

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AFFILIATION: Department of Biology, Seton Hall University, McNulty Hall, Room 215, South Orange, NJ 07079, USA.

Country: Netherlands

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MEDLINETA: Int Immunopharmacol

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