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Histochemical identification of methicillin-resistant Staphylococcus aureus: contribution to preventing nosocomial infection.

Histochemical identification of methicillin-resistant Staphylococcus aureus: contribution to preventing nosocomial infection. Research Abstract Details 

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  • Histochemical identification of methicillin-resistant Staphylococcus aureus: contribution to preventing nosocomial infection. Abstract Text:

    ryoichi shimomuraRyoichi Shimomura,yutaka tsutsumiYutaka Tsutsumi,ryoichi shimomuraRyoichi Shimomura,yutaka tsutsumiYutaka Tsutsumi,

    Histopathological diagnosis of bacterial infection remains a technical challenge. Application of histochemistry provides a prospect of the improvement of diagnostic quality. Methicillin-resistant Staphylococcus aureus (MRSA), the most common drug-resistant bacterium, is of clinical importance in making appropriate histopathological diagnosis. Recently, community-acquired MRSA has expanded, in addition to conventional hospital-acquired MRSA. Immunohistochemical identification of MRSA requires antibodies against both species-specific antigens and penicillin-binding protein 2' (PBP2'), whereas a species-specific gene and mec A encoding PBP2' can be the target of in situ hybridization (ISH) detection. Specificity verification in histochemistry should be emphasized, since S. aureus commonly expresses protein A in the cell wall, whose immunoglobulin-binding capacity is retrieved by heating pretreatment of routinely prepared sections. The ISH technique for MRSA needs meticulous pretreatment of routine paraffin-embedded sections and signal enhancement sequence. This review focuses on such histopathological approaches, which should have profound potential for contributing to decreasing and preventing nosocomial infection of MRSA.

    Histochemical identification of methicillin-resistant Staphylococcus aureus: contribution to preventing nosocomial infection. Publishing Authors By Initials

    r shimomuraR Shimomura,y tsutsumiY Tsutsumi,r shimomuraR Shimomura,y tsutsumiY Tsutsumi,

    For similar abstracts research abstracts see: abstracts research

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    Histochemical identification of methicillin-resistant Staphylococcus aureus: contribution to preventing nosocomial infection. Journal Published:

    PUBLICATION TYPE: Research Support, Non-U.S. Gov

    Journal: Seminars in diagnostic pathology

    VOLUME: 24

    Page Numbers: 217-26

    Journal Abbreviation: Semin Diagn Pathol

    ISSN: 0740-2570

    DAY: 18

    MONTH: Nov

    YEAR: 2007

    Histochemical identification of methicillin-resistant Staphylococcus aureus: contribution to preventing nosocomial infection. Information

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    LANGUAGE: eng

    NlmUniqueID: 8502262

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    Grant and Affiliation Information for Histochemical identification of methicillin-resistant Staphylococcus aureus: contribution to preventing nosocomial infection.

    AFFILIATION: Department of Pathology, Fujita Health University School of Medicine, Toyoake, Aichi 470-1192, Japan. rshimo@fujita-hu.ac.jp

    Country: United States

    United States Research PublicationUnited States Research Publication

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    MEDLINETA: Semin Diagn Pathol

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