Highly Efficient Enzymatic Synthesis of 2-Monoacylglycerides and Structured Lipids and their Production on a Technical Scale.

Highly Efficient Enzymatic Synthesis of 2-Monoacylglycerides and Structured Lipids and their Production on a Technical Scale. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Highly Efficient Enzymatic Synthesis of 2-Monoacylglycerides and Structured Lipids and their Production on a Technical Scale. Abstract Text:

Jan Pfeffer,Andreas Freund,Rachid Bel-Rhlid,Carl-Erik Hansen,Matthias Reuss,Rolf D Schmid,Steffen C Maurer,Jan Pfeffer,Andreas Freund,Rachid Bel-Rhlid,Carl-Erik Hansen,Matthias Reuss,Rolf D Schmid,Steffen C Maurer,Jan Pfeffer,Andreas Freund,Rachid Bel-Rhlid,Carl-Erik Hansen,Matthias Reuss,Rolf D Schmid,Steffen C Maurer,

We report here a two-step process for the high-yield enzymatic synthesis of 2-monoacylglycerides (2-MAG) of saturated as well as unsaturated fatty acids with different chain lengths. The process consists of two steps: first the unselective esterification of fatty acids and glycerol leading to a triacylglyceride followed by an sn1,3-selective alcoholysis reaction yielding 2-monoacylglycerides. Remarkably, both steps can be catalyzed by lipase B from Candida antarctica (CalB). The whole process including esterification and alcoholysis was scaled up in a miniplant to a total volume of 10 l. With this volume, a two-step process catalyzed by CalB for the synthesis of 1,3-oleoyl-2-palmitoylglycerol (OPO) using tripalmitate as starting material was established. On a laboratory scale, we obtained gram quantities of the synthesized 2-monoacylglycerides of polyunsaturated fatty acids such as arachidonic-, docosahexaenoic- and eicosapentaenoic acids and up to 96.4% of the theoretically possible yield with 95% purity. On a technical scale (>100 g of product, >5 l of reaction volume), 97% yield was reached in the esterification and 73% in the alcoholysis and a new promising process for the enzymatic synthesis of OPO was established.

Highly Efficient Enzymatic Synthesis of 2-Monoacylglycerides and Structured Lipids and their Production on a Technical Scale. Publishing Authors By Initials

J Pfeffer,A Freund,R Bel-Rhlid,CE Hansen,M Reuss,RD Schmid,SC Maurer,J Pfeffer,A Freund,R Bel-Rhlid,CE Hansen,M Reuss,RD Schmid,SC Maurer,J Pfeffer,A Freund,R Bel-Rhlid,CE Hansen,M Reuss,RD Schmid,SC Maurer,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Highly Efficient Enzymatic Synthesis of 2-Monoacylglycerides and Structured Lipids and their Production on a Technical Scale. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Lipids

VOLUME: 42

Page Numbers: 947-53

Journal Abbreviation: Lipids

ISSN: 0024-4201

DAY: 11

MONTH: 07

YEAR: 2007

Highly Efficient Enzymatic Synthesis of 2-Monoacylglycerides and Structured Lipids and their Production on a Technical Scale. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 60450

Highly Efficient Enzymatic Synthesis of 2-Monoacylglycerides and Structured Lipids and their Production on a Technical Scale. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Highly Efficient Enzymatic Synthesis of 2-Monoacylglycerides and Structured Lipids and their Production on a Technical Scale. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Highly Efficient Enzymatic Synthesis of 2-Monoacylglycerides and Structured Lipids and their Production on a Technical Scale.

AFFILIATION: Institute of Technical Biochemistry, University of Stuttgart, Allmandring 31, 70569, Stuttgart, Germany, steffen.maurer@itb.uni-stuttgart.de.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Lipids

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Highly Efficient Enzymatic Synthesis of 2-Monoacylglycerides and Structured Lipids and their Production on a Technical Scale Related Publications

• Acquired cold urticaria symptoms can be safely prevented by ebastine.
• Adaptive Designs in Clinical Drug Development: Opportunities, Challenges, and Scope Reflections Following PhRMA's November 2006 Workshop.
• Assessing combinations of cytotoxic agents using leukemia cell lines.
• Cell-permeable peptides induce dose- and length-dependent cytotoxic effects.
• Depression and functional impairment independently contribute to decreased quality of life in cancer patients prior to chemotherapy.
• Do All Patients with Community-acquired Pneumonia Need Antibiotics Within 4 Hours of Presentation?
• Early detection of schizophrenia: current evidence and future perspectives.
• Egg labeling methods for gastric emptying scintigraphy are not equivalent in producing a stable solid meal.
• Employing mutants to study thrombin residues responsible for factor XIII activation peptide recognition: a kinetic study.
• Exposure of bovine embryos to trypsin during washing does not decrease embryonic survival.
• Highly Efficient Enzymatic Synthesis of 2-Monoacylglycerides and Structured Lipids and their Production on a Technical Scale.
• Improving state pain policies: recent progress and continuing opportunities.
• Information fusion in biomedical image analysis: combination of data vs. combination of interpretations.
• Metabolism and toxicological detection of the designer drug 4-iodo-2,5-dimethoxy-amphetamine (DOI) in rat urine using gas chromatography-mass spectrometry.
• Modelling socioeconomic and health determinants of health-care use: a semiparametric approach.
• Nuclear medicine training for radiology residents: needs assessment survey.
• Oxybutynin for hyperhidrosis.
• Perturbations in factor XIII resulting from activation and inhibition examined by solution based methods and detected by MALDI-TOF MS.
• Platelet binding and biodistribution of [99mTc]rBitistatin in animal species and humans.
• Powerful short-cuts for multiple testing procedures with special reference to gatekeeping strategies.
• Preservation of microelastic properties of dentin and tooth enamel in vitro--a scanning acoustic microscopy study.
• Requestioning depression in patients with cancer: Contribution of somatic and affective symptoms to Beck's Depression Inventory.
• Retropubic surgery.
• Shape-based averaging.
• Superovulation and embryo quality in beef cows using PMSG and a monoclonal anti-PMSG.
• The complex morphology of Maurer's clefts: from discovery to three-dimensional reconstructions.
• Validated liquid chromatographic-electrospray ionization mass spectrometric assay for simultaneous determination of 3,4-methylenedioxymethamphetamine and its metabolites 3,4-methylenedioxyamphetamine, 3,4-dihydroxymethamphetamine, and 4-hydroxy-3-methoxym
• [In Process Citation]
• [Not Available.]
• [Not Available.]