High-titre antibodies to a foreign epitope elicited by affinity-purified hybrid LamB proteins.

High-titre antibodies to a foreign epitope elicited by affinity-purified hybrid LamB proteins. Research Abstract Details 

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High-titre antibodies to a foreign epitope elicited by affinity-purified hybrid LamB proteins. Abstract Text:

F Gargiulo,E Monti,A Caruso,N Manca,F Martinelli,C De Rango,G Flamminio,J Gao,A Preti,A Turano,

A monoclonal antibody to the LamB protein, named LBS-1, was developed and characterized. It was then covalently bound to Sepharose and used to purify hybrid LamB proteins from Escherichia coli crude extracts. A peptide of the interferon-gamma (IFN-gamma) NH2-terminal region, inserted within the LamB protein, was used as a model to assess immune response in mice injected with sonicated E. coli extract or with affinity-purified hybrid LamB protein. None of the mice immunized with the whole bacterial extract produced antibodies to IFN-gamma. On the other hand, all the mice immunized with the purified protein developed high-titre anti-IFN-gamma antibodies. These results might be due to the presence of bacterial components capable of masking the LamB protein to the immune system. The use of affinity-purified LamB proteins may constitute in some instances a more effective way of generating an immune response against foreign epitopes as opposed to whole bacterial antigens.

High-titre antibodies to a foreign epitope elicited by affinity-purified hybrid LamB proteins. Publishing Authors By Initials

F Gargiulo,E Monti,A Caruso,N Manca,F Martinelli,C De Rango,G Flamminio,J Gao,A Preti,A Turano,

For similar proteins: membrane proteins: receptors, cell surface: receptors, virus research abstracts see: proteins: membrane proteins: receptors, cell surface: receptors, virus research

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High-titre antibodies to a foreign epitope elicited by affinity-purified hybrid LamB proteins. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Vaccine

VOLUME: 11

Page Numbers: 1093-6

Journal Abbreviation: Vaccine

ISSN: 0264-410X

DAY: 27

MONTH: 11

YEAR: 1993

High-titre antibodies to a foreign epitope elicited by affinity-purified hybrid LamB proteins. Information

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LANGUAGE: eng

NlmUniqueID: 8406899

High-titre antibodies to a foreign epitope elicited by affinity-purified hybrid LamB proteins. Keywords Mesh Terms:

KEYWORDS: Receptors, Virus

MESH TERMS: isolation & purification

Chemical & Substance for Abstract: High-titre antibodies to a foreign epitope elicited by affinity-purified hybrid LamB proteins. Information

Substance Name: Interferon Type II

Registry Number: 82115-62-6

Grant and Affiliation Information for High-titre antibodies to a foreign epitope elicited by affinity-purified hybrid LamB proteins.

AFFILIATION: Institute of Microbiology, University of Brescia, Italy.

Country: ENGLAND

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MEDLINETA: Vaccine

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