High-throughput screening for functional adenosine to inosine RNA editing systems.

High-throughput screening for functional adenosine to inosine RNA editing systems. Research Abstract Details 

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High-throughput screening for functional adenosine to inosine RNA editing systems. Abstract Text:

Subhash Pokharel,Peter A Beal,

Deamination of adenosines within messenger RNAs catalyzed by adenosine deaminases that act on RNA (ADAR) enzymes generates inosines at the corresponding nucleotide positions. Because inosine is decoded as guanosine, this reaction can lead to codon changes and the introduction of amino acids into a gene product not encoded in the gene. Translation of the different coding strands created by this process leads to protein structural diversity in the parent organism and is necessary for nervous system function in metazoa. The basis for selective editing of adenosines within certain codons is not well understood at the structural/biochemical level. Here we describe a high-throughput screen for ADAR/substrate combinations capable of RNA editing that can be carried out in the yeast Saccharomyces cerevisiae growing on agar plates. Results from the screening of libraries of human ADAR2 mutants and libraries of RNA substrates shed light on structure-activity relationships in the ADAR-catalyzed adenosine to inosine RNA editing reaction.

High-throughput screening for functional adenosine to inosine RNA editing systems. Publishing Authors By Initials

S Pokharel,PA Beal,

For similar biochemical phenomena, metabolism, and nutrition: biochemical phenomena: substrate specificity research abstracts see: biochemical phenomena, metabolism, and nutrition: biochemical phenomena: substrate specificity research

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High-throughput screening for functional adenosine to inosine RNA editing systems. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: ACS chemical biology

VOLUME: 1

Page Numbers: 761-5

Journal Abbreviation: ACS Chem. Biol.

ISSN: 1554-8937

DAY: 15

MONTH: Dec

YEAR: 2006

High-throughput screening for functional adenosine to inosine RNA editing systems. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101282906

High-throughput screening for functional adenosine to inosine RNA editing systems. Keywords Mesh Terms:

KEYWORDS: Substrate Specificity

MESH TERMS: genetics

Chemical & Substance for Abstract: High-throughput screening for functional adenosine to inosine RNA editing systems. Information

Substance Name: Adenosine Deaminase

Registry Number: EC 3.5.4.4

Grant and Affiliation Information for High-throughput screening for functional adenosine to inosine RNA editing systems.

AFFILIATION:

Country: United States

AGENCY: United States NIGMS

GRANT: GM61115

ACRONYM: GM

MEDLINETA: ACS Chem Biol

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