High-throughput plasmid cDNA library screening.

High-throughput plasmid cDNA library screening. Research Abstract Details

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High-throughput plasmid cDNA library screening. Abstract Text:

Kenneth H Wan,Charles Yu,Reed A George,Joseph W Carlson,Roger A Hoskins,Robert Svirskas,Mark Stapleton,Susan E Celniker,Kenneth H Wan,Charles Yu,Reed A George,Joseph W Carlson,Roger A Hoskins,Robert Svirskas,Mark Stapleton,Susan E Celniker,

Libraries of cDNA clones are valuable resources for analyzing the expression, structure and regulation of genes, and for studying protein functions and interactions. Full-length cDNA clones provide information about intron and exon structures, splice junctions, and 5' and 3' untranslated regions (UTRs). Open reading frames (ORFs) derived from cDNA clones can be used to generate constructs allowing the expression of both wild-type proteins and proteins tagged at their amino or carboxy terminus. Thus, obtaining full-length cDNA clones and sequences for most or all genes in an organism is essential for understanding genome functions. EST sequencing samples cDNA libraries at random, an approach that is most useful at the beginning of large-scale screening projects. As projects progress towards completion, however, the probability of identifying unique cDNAs by EST sequencing diminishes, resulting in poor recovery of rare transcripts. Here we describe an adapted, high-throughput protocol intended for the recovery of specific, full-length clones from plasmid cDNA libraries in 5 d.

High-throughput plasmid cDNA library screening. Publishing Authors By Initials

KH Wan,C Yu,RA George,JW Carlson,RA Hoskins,R Svirskas,M Stapleton,SE Celniker,KH Wan,C Yu,RA George,JW Carlson,RA Hoskins,R Svirskas,M Stapleton,SE Celniker,

For similar genetic structures: plasmids research abstracts see: genetic structures: plasmids research

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High-throughput plasmid cDNA library screening. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Nature protocols

VOLUME: 1

Page Numbers: 624-32

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ISSN: 1750-2799

DAY: 3

MONTH: 12

YEAR: 2006

High-throughput plasmid cDNA library screening. Information

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LANGUAGE: eng

NlmUniqueID: 101284307

High-throughput plasmid cDNA library screening. Keywords Mesh Terms:

KEYWORDS: Plasmids

MESH TERMS: genetics

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Grant and Affiliation Information for High-throughput plasmid cDNA library screening.

AFFILIATION: Department of Genome Sciences, Earnest Orlando Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, California 94720, USA.

Country: England