High throughput microscopy: from raw images to discoveries.

High throughput microscopy: from raw images to discoveries. Research Abstract Details 

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High throughput microscopy: from raw images to discoveries. Abstract Text:

Roy Wollman,Nico Stuurman,Roy Wollman,Nico Stuurman,

Technological advances in automated microscopy now allow rapid acquisition of many images without human intervention, images that can be used for large-scale screens. The main challenge in such screens is the conversion of the raw images into interpretable information and hence discoveries. This post-acquisition component of image-based screens requires computational steps to identify cells, choose the cells of interest, assess their phenotype, and identify statistically significant ;hits'. Designing such an analysis pipeline requires careful consideration of the necessary hardware and software components, image analysis, statistical analysis and data presentation tools. Given the increasing availability of such hardware and software, these types of experiments have come within the reach of individual labs, heralding many interesting new ways of acquiring biological knowledge.

High throughput microscopy: from raw images to discoveries. Publishing Authors By Initials

R Wollman,N Stuurman,R Wollman,N Stuurman,

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High throughput microscopy: from raw images to discoveries. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of cell science

VOLUME: 120

Page Numbers: 3715-22

Journal Abbreviation: J. Cell. Sci.

ISSN: 0021-9533

DAY: 1

MONTH: Nov

YEAR: 2007

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LANGUAGE: eng

NlmUniqueID: 52457

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AFFILIATION: Department of Molecular and Cellular Biology, University of California, Davis, CA, USA.

Country: England

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MEDLINETA: J Cell Sci

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