High-throughput fluorescent multiplex array for indoor allergen exposure assessment.

High-throughput fluorescent multiplex array for indoor allergen exposure assessment. Research Abstract Details 

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High-throughput fluorescent multiplex array for indoor allergen exposure assessment. Abstract Text:

Christopher D Earle,Eva M King,Amy Tsay,Kimberly Pittman,Branka Saric,Lisa Vailes,Rebecca Godbout,Kerry G Oliver,Martin D Chapman,

BACKGROUND: Current enzyme immunoassay methods for detection of common indoor allergens in environmental dust samples are labor-intensive and time consuming. OBJECTIVE: To develop and validate a fluorescent multiplex array to measure 6 (Der p 1, Der f 1, Der p 2, Der f 2, Fel d 1, and Can f 1) indoor allergen levels simultaneously. METHODS: A multiplex array for 6 allergens, using mAbs covalently coupled to fluorescent microspheres, was developed using a single universal standard composed of purified natural allergens. The multiplex array was validated by comparing the measured dust mite, cat, and dog allergen levels in household dust samples to those obtained by standard ELISA methods. RESULTS: Linear regression analysis showed a highly significant quantitative correlation between the multiplex array and ELISA for dust mite, cat, and dog allergens: R(2) values ranging from 0.90 to 0.99 (P < .001). In addition, the sensitivity, limit of detection (<0.1 ng/mL), reproducibility, intra-assay coefficient of variance (<5%), and interassay coefficient of variance (<25%) of the fluorescent multiplex array were shown to be equal to or better than the ELISA method. CONCLUSION: A multiplex array has been developed to measure simultaneously 6 indoor allergens from a single sample. The array will facilitate epidemiologic studies and indoor air quality assessments and can, in principle, be expanded to include other allergens and biologics. CLINICAL IMPLICATIONS: The multiplex array lends itself to clinical studies, population-based environmental surveys, and allergen avoidance studies comparing allergen exposure in large populations over several time points.

High-throughput fluorescent multiplex array for indoor allergen exposure assessment. Publishing Authors By Initials

CD Earle,EM King,A Tsay,K Pittman,B Saric,L Vailes,R Godbout,KG Oliver,MD Chapman,

For similar investigative techniques: epidemiologic methods: statistics as topic: sensitivity and specificity research abstracts see: investigative techniques: epidemiologic methods: statistics as topic: sensitivity and specificity research

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High-throughput fluorescent multiplex array for indoor allergen exposure assessment. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: The Journal of allergy and clinical immunology

VOLUME: 119

Page Numbers: 428-33

Journal Abbreviation: J. Allergy Clin. Immunol.

ISSN: 0091-6749

DAY: 27

MONTH: 12

YEAR: 2006

High-throughput fluorescent multiplex array for indoor allergen exposure assessment. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 1275002

High-throughput fluorescent multiplex array for indoor allergen exposure assessment. Keywords Mesh Terms:

KEYWORDS: Sensitivity and Specificity

MESH TERMS: methods

Chemical & Substance for Abstract: High-throughput fluorescent multiplex array for indoor allergen exposure assessment. Information

Substance Name: Allergens

Registry Number: 0

Grant and Affiliation Information for High-throughput fluorescent multiplex array for indoor allergen exposure assessment.

AFFILIATION: Indoor Biotechnologies, Inc, Charlottesville, VA 22903, USA. cearle@inbio.com <cearle@inbio.com>

Country: United States

AGENCY: United States NIEHS

GRANT: ES 55545

ACRONYM: ES

MEDLINETA: J Allergy Clin Immunol

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