High throughput cryopreservation of 140,000 Physcomitrella patens mutants.

High throughput cryopreservation of 140,000 Physcomitrella patens mutants. Research Abstract Details 

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High throughput cryopreservation of 140,000 Physcomitrella patens mutants. Abstract Text:

J Schulte,R Reski,

A high throughput protocol was established to preserve 140,000 mutants of a moss, Physcomitrella patens, a model plant for functional genomics studies, over liquid nitrogen. Regarding the reliable long-term storage of diverse mutant phenotypes, as well as time and cost effectiveness, each working step was optimized: 1) plant preparation, 2) freezing regime, cryogenic conditions, 3) regrowth after thawing. A prerequisite for maximum regrowth was a 1-week preculture of chopped plant material on a supplemented medium prior to freezing. Cryo vials as preculture vessels resulted in identical regrowth rates, compared to petri dishes. The cryo vial type had a significant influence on regrowth rates. A cooling rate of - 1 degrees C/min down to - 35 degrees C with a 10 min holding time before transferring plants to - 152 degrees C was the most suitable freezing regime. This protocol allows a cryopreservation of 1100 plants during a 5-day working week, practicable by one person. For more than 650 cryopreserved mutants a maximum regrowth rate of 100 % was obtained, independently of mutant phenotypes.

High throughput cryopreservation of 140,000 Physcomitrella patens mutants. Publishing Authors By Initials

J Schulte,R Reski,

For similar genetic phenomena: variation (genetics): mutation research abstracts see: genetic phenomena: variation (genetics): mutation research

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MEDLINE DATE: 2004 Mar-Apr

High throughput cryopreservation of 140,000 Physcomitrella patens mutants. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Plant biology (Stuttgart, Germany)

VOLUME: 6

Page Numbers: 119-27

Journal Abbreviation: Plant Biol (Stuttg)

ISSN: 1435-8603

DAY: 7

MONTH: 01

YEAR: 2008

High throughput cryopreservation of 140,000 Physcomitrella patens mutants. Information

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LANGUAGE: eng

NlmUniqueID: 101148926

High throughput cryopreservation of 140,000 Physcomitrella patens mutants. Keywords Mesh Terms:

KEYWORDS: Mutation

MESH TERMS: methods

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Substance Name: Culture Media

Registry Number: 0

Grant and Affiliation Information for High throughput cryopreservation of 140,000 Physcomitrella patens mutants.

AFFILIATION: Plant Biotechnology, Freiburg University, Freiburg, Germany.

Country: Germany

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MEDLINETA: Plant Biol (Stuttg)

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