High-resolution studies of lung surfactant collapse.

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High-resolution studies of lung surfactant collapse. Abstract Text:

Rachel Sibug-Aga,Robert C Dunn,

Survanta is a replacement lung surfactant (LS) used in the treatment of respiratory distress syndrome (RDS), the fourth leading cause of infant mortality in the United States. It consists of purified LS from bovine sources and retains the surfactant proteins (SP) SP-B and SP-C, both thought to be important in proper respiratory function. As such, it provides a useful and biologically relevant model system to probe the structure and function of natural LS. Here, we report results from high-resolution studies on model monolayers formed from Survanta to probe the mechanism of collapse at high surface pressure. Our results show the formation of two different collapse structures. At 62 mN/m, slightly below the collapse pressure, monolayer collapse occurs through buckling. Confocal fluorescence measurements on supported films reveal regions of overlapping phase structure in the films that mark the transition from monolayer to multilayer. Simultaneous near-field scanning optical microscopy fluorescence and force measurements show that the transition seen in the fluorescence measurements accompanies corresponding approximately 4-5 nm changes in membrane topography. This change in height is consistent with bilayer formation on monolayer collapse. Analysis of the phase structure near the transitions also suggests that the buckling occurs from a continuous film. However, when the film is compressed to its collapse pressure of 65 mN/m, buckling is no longer evident in the collapsed region. In addition, multilayers and lipid-protein aggregates that are up to 40 nm higher than the monolayer are observed in the collapsed film at this pressure.

High-resolution studies of lung surfactant collapse. Publishing Authors By Initials

R Sibug-Aga,RC Dunn,

For similar chemical actions and uses: pharmacologic actions: therapeutic uses: respiratory system agents: pulmonary surfactants research abstracts see: chemical actions and uses: pharmacologic actions: therapeutic uses: respiratory system agents: pulmonary surfactants research

PUBMED ID PMID:

MEDLINE DATE: 2004 Nov-Dec

High-resolution studies of lung surfactant collapse. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Photochemistry and photobiology

VOLUME: 80

Page Numbers: 471-6

Journal Abbreviation: Photochem. Photobiol.

ISSN: 0031-8655

DAY: 3

MONTH: 12

YEAR: 2007

High-resolution studies of lung surfactant collapse. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376425

High-resolution studies of lung surfactant collapse. Keywords Mesh Terms:

KEYWORDS: Pulmonary Surfactants

MESH TERMS: pharmacology

Chemical & Substance for Abstract: High-resolution studies of lung surfactant collapse. Information

Substance Name: beractant

Registry Number: 108778-82-1

Grant and Affiliation Information for High-resolution studies of lung surfactant collapse.

AFFILIATION: Department of Chemistry, University of Kansas, Lawrence, KS 66045, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: GM55290

ACRONYM: GM

MEDLINETA: Photochem Photobiol

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