High resolution structure and catalysis of O-acetylserine sulfhydrylase isozyme B from Escherichia coli.

High resolution structure and catalysis of O-acetylserine sulfhydrylase isozyme B from Escherichia coli. Research Abstract Details 

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High resolution structure and catalysis of O-acetylserine sulfhydrylase isozyme B from Escherichia coli. Abstract Text:

Georg Zocher,Ulrich Wiesand,Georg E Schulz,Georg Zocher,Ulrich Wiesand,Georg E Schulz,

The crystal structure of the dimeric O-acetylserine sulfhydrylase isozyme B from Escherichia coli (CysM), complexed with the substrate analog citrate, has been determined at 1.33 A resolution by X-ray diffraction analysis. The C1-carboxylate of citrate was bound at the carboxylate position of O-acetylserine, whereas the C6-carboxylate adopted two conformations. The activity of the enzyme and of several active center mutants was determined using an assay based on O-acetylserine and thio-nitrobenzoate (TNB). The unnatural substrate TNB was modeled into the reported structure. The substrate model and the observed mutant activities may facilitate future protein engineering attempts designed to broaden the substrate spectrum of the enzyme. A comparison of the reported structure with previously published CysM structures revealed large conformational changes. One of the crystal forms contained two dimers, each of which comprised one subunit in a closed and one in an open conformation. Although the homodimer asymmetry was most probably caused by crystal packing, it indicates that the enzyme can adopt such a state in solution, which may be relevant for the catalytic reaction.

High resolution structure and catalysis of O-acetylserine sulfhydrylase isozyme B from Escherichia coli. Publishing Authors By Initials

G Zocher,U Wiesand,GE Schulz,G Zocher,U Wiesand,GE Schulz,

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High resolution structure and catalysis of O-acetylserine sulfhydrylase isozyme B from Escherichia coli. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: The FEBS journal

VOLUME: 274

Page Numbers: 5382-9

Journal Abbreviation: FEBS J.

ISSN: 1742-464X

DAY: 26

MONTH: 09

YEAR: 2007

High resolution structure and catalysis of O-acetylserine sulfhydrylase isozyme B from Escherichia coli. Information

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LANGUAGE: eng

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Grant and Affiliation Information for High resolution structure and catalysis of O-acetylserine sulfhydrylase isozyme B from Escherichia coli.

AFFILIATION: Institut für Organische Chemie und Biochemie, Albert-Ludwigs-Universität, Freiburg im Breisgau, Germany.

Country: England

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MEDLINETA: FEBS J

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