High-performance thin-layer chromatography method for quantitative determination of oenothein B and quercetin glucuronide in aqueous extract of Epilobii angustifolii herba.

High-performance thin-layer chromatography method for quantitative determination of oenothein B and quercetin glucuronide in aqueous extract of Epilobii angustifolii herba. Research Abstract Details 

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High-performance thin-layer chromatography method for quantitative determination of oenothein B and quercetin glucuronide in aqueous extract of Epilobii angustifolii herba. Abstract Text:

Agnieszka Bazylko,Anna K Kiss, Kowalski,Agnieszka Bazylko,Anna K Kiss, Kowalski,Agnieszka Bazylko,Anna K Kiss, Kowalski,

A method was developed for separation and quantitative determination of oenothein B (OeB) and quercetin glucuronide (QG) in aqueous extract of Epilobii angustifolii herba by HPTLC-densitometry. The analyses were performed on HPTLC RP-18 WF(254) plates with 25% MeCN in water (+50mM H(3)PO(4)) as the mobile phase (distance of 8cm) for OeB quantification and then with acetonitrile (distance of 4cm) for QG quantification. OeB and QG were determined by densitometry at 270 and 350nm, respectively. Their amounts were calculated using the regression equations of the calibration curves which were linear in a range of 1.14-2.28mugspot(-1) for OeB and of 0.0768-0.6912mugspot(-1) for QG. The amounts of OeB and QG in aqueous extract of Epilobii angustifolii herba measured by the method developed were 152.46+/-4.92 and 22.07+/-1.38mgg(-1), respectively. The method was found to be relatively simple, specific, precise and accurate for the quality control of Epilobium angustifolium extracts.

High-performance thin-layer chromatography method for quantitative determination of oenothein B and quercetin glucuronide in aqueous extract of Epilobii angustifolii herba. Publishing Authors By Initials

A Bazylko,AK Kiss,J Kowalski,A Bazylko,AK Kiss,J Kowalski,A Bazylko,AK Kiss,J Kowalski,

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High-performance thin-layer chromatography method for quantitative determination of oenothein B and quercetin glucuronide in aqueous extract of Epilobii angustifolii herba. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of chromatography. A

VOLUME: 1173

Page Numbers: 146-50

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ISSN: 0021-9673

DAY: 12

MONTH: 10

YEAR: 2007

High-performance thin-layer chromatography method for quantitative determination of oenothein B and quercetin glucuronide in aqueous extract of Epilobii angustifolii herba. Information

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LANGUAGE: eng

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Grant and Affiliation Information for High-performance thin-layer chromatography method for quantitative determination of oenothein B and quercetin glucuronide in aqueous extract of Epilobii angustifolii herba.

AFFILIATION: Department of Pharmacognosy and Molecular Basis of Phytotherapy, Faculty of Pharmacy, Warsaw Medical University, ul. Banacha 1, 02-097 Warszawa, Poland.

Country: Netherlands

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MEDLINETA: J Chromatogr A

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