High performance liquid chromatography was performed by the ion pair method on unsaturated disaccharides produced from chondroitin sulfates by the action of chondroitinase. Completely separated peaks corresponding to deltaDi-0S, deltaDi-4S, and deltaDi-6S were obtained on a column of mu-bondapak-C18 with 0.035 M tetra-butylammonium phosphate (pH 7.54) as a mobile phase. There was a linear relationship between the ratio of the peak area and the amount of each standard tested.
High performance liquid chromatography of unsaturated disaccharides produced from chondroitin sulfates by chondroitinase. Publishing Authors By Initials
High performance liquid chromatography of unsaturated disaccharides produced from chondroitin sulfates by chondroitinase. Journal Published:
PUBLICATION TYPE: Journal Article
Journal: Journal of biochemistry
VOLUME: 85
Page Numbers: 1383-5
Journal Abbreviation: J. Biochem.
ISSN: 0021-924X
DAY: 19
MONTH: May
YEAR: 1979
High performance liquid chromatography of unsaturated disaccharides produced from chondroitin sulfates by chondroitinase. Information
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LANGUAGE: eng
NlmUniqueID: 376600
High performance liquid chromatography of unsaturated disaccharides produced from chondroitin sulfates by chondroitinase. Keywords Mesh Terms:
KEYWORDS: Disaccharides
MESH TERMS: isolation & purification
Chemical & Substance for Abstract: High performance liquid chromatography of unsaturated disaccharides produced from chondroitin sulfates by chondroitinase. Information
Substance Name: Chondroitinases and Chondroitin Lyases
Registry Number: EC 4.2.2.-
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Country: JAPAN
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MEDLINETA: J Biochem
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