High mucosal serotonin availability in neonatal guinea pig ileum is associated with low serotonin transporter expression.

High mucosal serotonin availability in neonatal guinea pig ileum is associated with low serotonin transporter expression. Research Abstract Details 

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High mucosal serotonin availability in neonatal guinea pig ileum is associated with low serotonin transporter expression. Abstract Text:

Xiaochun Bian,Bhavik Patel,Xiaoling Dai,James J Galligan,Greg Swain,

BACKGROUND & AIMS: 5-Hydroxytryptamine (5-HT) is a neurotransmitter and paracrine signaling molecule in the gut. Paracrine signaling by enterochromaffin cells (EC), which release 5-HT, has not been studied in neonates. Our aim was to compare 5-HT disposition in the intestinal mucosa of neonatal and adult guinea pigs. METHODS: 5-HT was locally measured in vitro from intestinal segments using a diamond microelectrode and continuous amperometry. The serotonin transporter (SERT) was measured using immunohistochemical and Western blot techniques. 5-HT intestinal content was measured using immunohistochemistry and high-performance liquid chromatography with electrochemical detection. RESULTS: An oxidation current, reflective of local 5-HT release, was recorded with the microelectrode near the mucosal surface, and this current was larger in neonatal than in adult tissues. Mechanically stimulating the mucosa with a fine glass probe evoked an additional current in adult but not neonatal tissues. Oxidation currents were reduced by tetrodotoxin and were blocked in calcium-free solutions. Fluoxetine (1 microM) potentiated oxidation currents in adult but not neonatal tissues. SERT levels were lower in neonatal vs adult tissues. There was no difference in 5-HT content between neonates and adults but 5-hydroxyindole acetic acid/5-HT ratios were higher in adults. EC cell counts showed no difference in cell number, but EC cells were found in the crypts in neonatal and along the villi in adult tissues. CONCLUSIONS: SERT expression is low in neonates, and this is associated with high levels of free mucosal 5-HT and reduced metabolism. Postnatal maturation of 5-HT signaling may be important for development of neurohumoral control of intestinal motor reflexes.

High mucosal serotonin availability in neonatal guinea pig ileum is associated with low serotonin transporter expression. Publishing Authors By Initials

X Bian,B Patel,X Dai,JJ Galligan,G Swain,

For similar heterocyclic compounds: heterocyclic compounds, 2-ring: quinazolines: tetrodotoxin research abstracts see: heterocyclic compounds: heterocyclic compounds, 2-ring: quinazolines: tetrodotoxin research

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High mucosal serotonin availability in neonatal guinea pig ileum is associated with low serotonin transporter expression. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Gastroenterology

VOLUME: 132

Page Numbers: 2438-47

Journal Abbreviation: Gastroenterology

ISSN: 0016-5085

DAY: 13

MONTH: 04

YEAR: 2007

High mucosal serotonin availability in neonatal guinea pig ileum is associated with low serotonin transporter expression. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 374630

High mucosal serotonin availability in neonatal guinea pig ileum is associated with low serotonin transporter expression. Keywords Mesh Terms:

KEYWORDS: Tetrodotoxin

MESH TERMS: pharmacology

Chemical & Substance for Abstract: High mucosal serotonin availability in neonatal guinea pig ileum is associated with low serotonin transporter expression. Information

Substance Name: Calcium

Registry Number: 7440-70-2

Grant and Affiliation Information for High mucosal serotonin availability in neonatal guinea pig ileum is associated with low serotonin transporter expression.

AFFILIATION: Department of Pharmacology & Toxicology, Michigan State University, East Lansing, Michigan 48824, USA.

Country: United States

AGENCY: United States NHLBI

GRANT: HL084258

ACRONYM: HL

MEDLINETA: Gastroenterology

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