High-level expression of recombinant active human renin in Sf-9 cells: rapid purification and characterization.

High-level expression of recombinant active human renin in Sf-9 cells: rapid purification and characterization. Research Abstract Details 

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High-level expression of recombinant active human renin in Sf-9 cells: rapid purification and characterization. Abstract Text:

Saori Takahashi,Keishi Hata,Ken-Ichi Kikuchi,Takeshi Gotoh,Saori Takahashi,Keishi Hata,Ken-Ichi Kikuchi,Takeshi Gotoh,

Recombinant human (rh) renin was expressed in Sf-9 insect cells. Baculovirus-infected Sf-9 cells produced active rh-renin in the late stage of cultivation. The rh-renin was purified after 5 d of culture by two steps of column chromatography. Approximately 0.61 mg of pure rh-renin was obtained from 200 ml of culture medium with a yield of 35.3%.

High-level expression of recombinant active human renin in Sf-9 cells: rapid purification and characterization. Publishing Authors By Initials

S Takahashi,K Hata,K Kikuchi,T Gotoh,S Takahashi,K Hata,K Kikuchi,T Gotoh,

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High-level expression of recombinant active human renin in Sf-9 cells: rapid purification and characterization. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Bioscience, biotechnology, and biochemistry

VOLUME: 71

Page Numbers: 2610-3

Journal Abbreviation: Biosci. Biotechnol. Biochem.

ISSN: 0916-8451

DAY: 7

MONTH: 10

YEAR: 2007

High-level expression of recombinant active human renin in Sf-9 cells: rapid purification and characterization. Information

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LANGUAGE: eng

NlmUniqueID: 9205717

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AFFILIATION: Institute for Food and Brewing, Akita Prefectural Agricultural, Forestry, and Fisheries Research Center, Japan. saori@arif.pref.akita.jp

Country: Japan

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MEDLINETA: Biosci Biotechnol Biochem

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