High glucose up-regulates lipopolysaccharide-stimulated inflammatory cytokine production via c-jun N-terminal kinase in the monocytic cell line THP-1.

High glucose up-regulates lipopolysaccharide-stimulated inflammatory cytokine production via c-jun N-terminal kinase in the monocytic cell line THP-1. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

High glucose up-regulates lipopolysaccharide-stimulated inflammatory cytokine production via c-jun N-terminal kinase in the monocytic cell line THP-1. Abstract Text:

Hirotaka Iwata,Yoshihiko Soga,Michio Meguro,Sayuri Yoshizawa,Yuka Okada,Yoshihiro Iwamoto,Akiko Yamashita,Shogo Takashiba,Fusanori Nishimura,

Diabetic subjects are susceptible to atherosclerosis. It has been postulated that inflammation plays a crucial role in atherogenesis. Since previous studies suggested persistent low-grade infection by Gram-negative bacteria such as Chlamydia spp. and/or periodontal infection is associated with increased atherogenesis among diabetic subjects, we hypothesized that macrophages under hyperglycemia respond to lipopolysaccharide (LPS) challenge in a more exaggerated manner than under normal glucose conditions. Therefore, we examined cytokine productivity and associated signal transduction molecules in LPS-stimulated the monocytic cell line THP-1, under conditions of hyperglycemia. Differentiated THP-1 cells were cultured under normal and high glucose conditions without fetal bovine serum, and were stimulated with Escherichia coli LPS in the presence of LPS binding protein. Following stimulation, activated signal transduction molecules were detected by protein microarray and confirmed thereafter. Results indicated that c-jun N-terminal kinase (JNK) was highly-phosphorylated at high glucose concentrations, and this was confirmed by Western-immunoblotting. Tumor necrosis factor-alpha and monocyte chemo-attractant protein-1 production were significantly enhanced under these conditions. SP600125, a selective inhibitor of JNK, dose-dependently suppressed the production of these cytokine. Therefore, we suggest that this may be one of the mechanisms by which sub-clinical infection by Gram-negative bacteria promotes atherosclerosis in diabetic subjects.

High glucose up-regulates lipopolysaccharide-stimulated inflammatory cytokine production via c-jun N-terminal kinase in the monocytic cell line THP-1. Publishing Authors By Initials

H Iwata,Y Soga,M Meguro,S Yoshizawa,Y Okada,Y Iwamoto,A Yamashita,S Takashiba,F Nishimura,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

High glucose up-regulates lipopolysaccharide-stimulated inflammatory cytokine production via c-jun N-terminal kinase in the monocytic cell line THP-1. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of endotoxin research

VOLUME: 13

Page Numbers: 227-34

Journal Abbreviation: J. Endotoxin Res.

ISSN: 0968-0519

DAY: 24

MONTH: 10

YEAR: 2007

High glucose up-regulates lipopolysaccharide-stimulated inflammatory cytokine production via c-jun N-terminal kinase in the monocytic cell line THP-1. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9433350

High glucose up-regulates lipopolysaccharide-stimulated inflammatory cytokine production via c-jun N-terminal kinase in the monocytic cell line THP-1. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: High glucose up-regulates lipopolysaccharide-stimulated inflammatory cytokine production via c-jun N-terminal kinase in the monocytic cell line THP-1. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for High glucose up-regulates lipopolysaccharide-stimulated inflammatory cytokine production via c-jun N-terminal kinase in the monocytic cell line THP-1.

AFFILIATION: Department of Pathophysiology - Periodontal Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.

Country: England

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Endotoxin Res

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

High glucose up-regulates lipopolysaccharide-stimulated inflammatory cytokine production via c-jun N-terminal kinase in the monocytic cell line THP-1 Related Publications

• A New Phenolic Glucoside, Curculigoside from Rhizomes of Curculigo orchioides.
• Action of ascorbic acid on a myosin molecule derived from carp.
• Alginate hydrogel linked with synthetic oligopeptide derived from BMP-2 allows ectopic osteoinduction in vivo.
• Analysis of N-glycan in serum glycoproteins from db/db mice and humans with type 2 diabetes.
• Arteriovenous malformation of the gallbladder: CT and angiographic findings.
• Combination tumor immunotherapy with radiotherapy and Th1 cell therapy against murine lung carcinoma.
• Continence care for older people.
• Dynamic memorization characteristics in neural networks with different neuronal dynamics.
• Evolution of life-history traits collapses competitive coexistence.
• Fluid-attenuated inversion-recovery SSFP imaging.
• Hemodynamics of ovarian veins: MR angiography in women with uterine leiomyomata.
• High glucose up-regulates lipopolysaccharide-stimulated inflammatory cytokine production via c-jun N-terminal kinase in the monocytic cell line THP-1.
• High peak inspiratory flow can aggravate ventilator-induced lung injury in rabbits.
• Influence of human CD8 on antigen recognition by T-cell receptor-transduced cells.
• Knockout of glutelin genes which form a tandem array with a high level of homology in rice by gamma irradiation.
• Management of urethral hemangiomas associated with Klippel-Trenaunay-Weber syndrome by endoscopic sclerotherapy.
• Multitensor tractography enables better depiction of motor pathways: initial clinical experience using diffusion-weighted MR imaging with standard b-value.
• PET measurement of FK506 concentration in a monkey model of stroke.
• Pharmacokinetics of telithromycin using bronchoscopic microsampling after single and multiple oral doses.
• Pitfall of percutaneous cardiopulmonary support for acute massive pulmonary embolism.
• Polarization of Healpha radiation due to anisotropy of fast-electron transport in ultraintense-laser-produced plasmas.
• Randomized placebo-controlled trial of interferon alpha-2b plus ribavirin with and without lactoferrin for chronic hepatitis C.
• Resilience, family adversity and well-being among Hawaiian and non-Hawaiian adolescents.
• Short term prediction of Ht value using BPNN during LCAP.
• Some characteristics of fluoride-induced cell death in rat thymocytes: cytotoxicity of sodium fluoride.
• Sternum fracture and haemorrhage after cardiopulmonary resuscitation.
• T cell immunotherapy.
• Three-dimensional bone-implant integration profiling using micro-computed tomography.
• Tumour necrosis factor alpha production by polymorphonuclear neutrophils treated with mouse anti-human CD19 monoclonal antibody.
• [Recent progress in paroxysmal nocturnal hemoglobinuria]