High-frequency S-layer protein variation in Campylobacter fetus revealed by sapA mutagenesis.

High-frequency S-layer protein variation in Campylobacter fetus revealed by sapA mutagenesis. Research Abstract Details 

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High-frequency S-layer protein variation in Campylobacter fetus revealed by sapA mutagenesis. Abstract Text:

Campylobacter fetus utilizes paracrystalline surface (S-) layer proteins that confer complement resistance and that undergo antigenic variation to facilitate persistent mucosal colonization in ungulates. C. fetus possesses multiple homologues of sapA, each of which encode full-length S-layer proteins. Disruption of sapA by a gene targeting method (insertion of kanamycin (km) resistance) caused the loss of C. fetus cells bearing full-length S-layer proteins and their replacement by cells bearing a 50 kDa truncated protein that was not exported to the cell surface. After incubation of the mutants with serum, the survival rate was approximately 2 x 10(-2). Immunoblots of survivors showed that phenotypic reversion involving high-level production of full-length (98, 127 or 149 kDa) S-layer proteins had occurred. Revertants were serum resistant but caused approximately 10-fold less bacteraemia in orally challenged mice than did the wild-type strain. Southern hybridizations of the revertants showed rearrangement of sapA homologues and retention of the km marker. These results indicate that there exists high-frequency generation of C. fetus sapA antigenic variants, and that intracellular mechanisms acting at the level of DNA reciprocal recombination play key roles in this phenomenon.

High-frequency S-layer protein variation in Campylobacter fetus revealed by sapA mutagenesis. Publishing Authors By Initials

For similar genetic processes: mutagenesis research abstracts see: genetic processes: mutagenesis research

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High-frequency S-layer protein variation in Campylobacter fetus revealed by sapA mutagenesis. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Molecular microbiology

VOLUME: 14

Page Numbers: 453-62

Journal Abbreviation: Mol. Microbiol.

ISSN: 0950-382X

DAY: 24

MONTH: Nov

YEAR: 1994

High-frequency S-layer protein variation in Campylobacter fetus revealed by sapA mutagenesis. Information

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LANGUAGE: eng

NlmUniqueID: 8712028

High-frequency S-layer protein variation in Campylobacter fetus revealed by sapA mutagenesis. Keywords Mesh Terms:

KEYWORDS: Mutagenesis

MESH TERMS: genetics

Chemical & Substance for Abstract: High-frequency S-layer protein variation in Campylobacter fetus revealed by sapA mutagenesis. Information

Substance Name: Complement System Proteins

Registry Number: 9007-36-7

Grant and Affiliation Information for High-frequency S-layer protein variation in Campylobacter fetus revealed by sapA mutagenesis.

AFFILIATION: Department of Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee 37232.

Country: ENGLAND

AGENCY: United States NIAID

GRANT: R01AI24145

ACRONYM: AI

MEDLINETA: Mol Microbiol

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