Hexacyanochromate ion as a paramagnetic anion probe for active sites of enzymes. Application to ribonuclease A.

Hexacyanochromate ion as a paramagnetic anion probe for active sites of enzymes. Application to ribonuclease A. Research Abstract Details 

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Hexacyanochromate ion as a paramagnetic anion probe for active sites of enzymes. Application to ribonuclease A. Abstract Text:

F Inagaki,K Watanabe,T Miyazawa,

The 270 MHZ proton MNR spectra of an aqueous solution of ribonuclease A in the presence of hexacyanochromate ions were measured. The C2 and C4 proton signals of the His 12 and His 119 residues were selectively broadened, indicating that this ion was bound to the active site. From analyses of concentration dependences of diamagnetic shifts, one hexacyanocobaltate ion was found to bind to the active site, with the dissociation constant of 8 mM. The paramagnetic hexacyanochromate ion is useful for mapping the active sites of enzymes for anionic substrates.

Hexacyanochromate ion as a paramagnetic anion probe for active sites of enzymes. Application to ribonuclease A. Publishing Authors By Initials

F Inagaki,K Watanabe,T Miyazawa,

For similar enzymes and coenzymes: enzymes: hydrolases: esterases: ribonucleases research abstracts see: enzymes and coenzymes: enzymes: hydrolases: esterases: ribonucleases research

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Hexacyanochromate ion as a paramagnetic anion probe for active sites of enzymes. Application to ribonuclease A. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 86

Page Numbers: 591-4

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Aug

YEAR: 1979

Hexacyanochromate ion as a paramagnetic anion probe for active sites of enzymes. Application to ribonuclease A. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Hexacyanochromate ion as a paramagnetic anion probe for active sites of enzymes. Application to ribonuclease A. Keywords Mesh Terms:

KEYWORDS: Ribonucleases

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Chemical & Substance for Abstract: Hexacyanochromate ion as a paramagnetic anion probe for active sites of enzymes. Application to ribonuclease A. Information

Substance Name: Ribonucleases

Registry Number: EC 3.1.-

Grant and Affiliation Information for Hexacyanochromate ion as a paramagnetic anion probe for active sites of enzymes. Application to ribonuclease A.

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Country: JAPAN

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MEDLINETA: J Biochem

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