[Heterotopic osteogenesis of autogenous marrow stromal cells with recombinant human bone morphogenetic protein 2 gene transfection and porous calcium phosphate ceramic as a scaffold]

[Heterotopic osteogenesis of autogenous marrow stromal cells with recombinant human bone morphogenetic protein 2 gene transfection and porous calcium phosphate ceramic as a scaffold] Research Abstract Details 

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[Heterotopic osteogenesis of autogenous marrow stromal cells with recombinant human bone morphogenetic protein 2 gene transfection and porous calcium phosphate ceramic as a scaffold] Abstract Text:

Xizhe Chen,Lianjia Yang,Weidong Tian,

OBJECTIVE: To observe the heterotopic osteogenesis of autogenous marrow stromal cells loading on porous calcium phosphate ceramic scaffolds with rhBMP2 gene transfection in a Sprague-Dawley rat model. METHODS: Autogenous marrow stromal cells were obtained from left femurs and tibias of 20 male adult SD rats under general anesthesia and sterile condition and cultured in alpha-Minimal Essential Medium supplemented with 15% fetal bovine serum. RhBMP2 gene was transfected into stromal cells by means of LipofectAMINE 2000 reagent five days after primary culture. The stably gene expressive cells were selected with G-418 for 14 days and mixed with stromal cells without transfection. The mixture cells were seeded and subcultured for another 10 days in porous calcium phosphate bioceramic that had been subjected to surface-modification via soaking in human plasma fibronectin. The cell-ceramic compound was implanted subcutaneously and intramuscularly in the corresponding rat. Lab animals were sacrificed at two-week intervals till twenty weeks postoperatively and the involved samples were removed. RESULTS: Morphologic and histological study demonstrated that cell-ceramic compound had an ability of heterotopic osteogenesis, which was similar to that of autogenous osteoblasts in previous study. CONCLUSION: It seems that autogenous stromal cells with rhBMP2 transfection acts as a bioreactor promoting proliferation and differentiation of stem cells when they are replanted into the corresponding animals.

[Heterotopic osteogenesis of autogenous marrow stromal cells with recombinant human bone morphogenetic protein 2 gene transfection and porous calcium phosphate ceramic as a scaffold] Publishing Authors By Initials

X Chen,L Yang,W Tian,

For similar peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta research abstracts see: peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta research

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[Heterotopic osteogenesis of autogenous marrow stromal cells with recombinant human bone morphogenetic protein 2 gene transfection and porous calcium phosphate ceramic as a scaffold] Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yi

VOLUME: 21

Page Numbers: 419-21

Journal Abbreviation:

ISSN: 1000-1182

DAY: 19

MONTH: Dec

YEAR: 2003

[Heterotopic osteogenesis of autogenous marrow stromal cells with recombinant human bone morphogenetic protein 2 gene transfection and porous calcium phosphate ceramic as a scaffold] Information

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LANGUAGE: chi

NlmUniqueID: 9422648

[Heterotopic osteogenesis of autogenous marrow stromal cells with recombinant human bone morphogenetic protein 2 gene transfection and porous calcium phosphate ceramic as a scaffold] Keywords Mesh Terms:

KEYWORDS: Transforming Growth Factor beta

MESH TERMS: physiology

Chemical & Substance for Abstract: [Heterotopic osteogenesis of autogenous marrow stromal cells with recombinant human bone morphogenetic protein 2 gene transfection and porous calcium phosphate ceramic as a scaffold] Information

Substance Name: calcium phosphate

Registry Number: 10103-46-5

Grant and Affiliation Information for [Heterotopic osteogenesis of autogenous marrow stromal cells with recombinant human bone morphogenetic protein 2 gene transfection and porous calcium phosphate ceramic as a scaffold]

AFFILIATION: Key Lab. of Oral Biomedical Engineering, Sichuan University, Ministry of Education, Chengdu 610041, China.

Country: China

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MEDLINETA: Hua Xi Kou Qiang Yi Xue Za Zhi

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