Heterologous expression of a gene encoding a thermostable beta-galactosidase from Alicyclobacillus acidocaldarius.

Heterologous expression of a gene encoding a thermostable beta-galactosidase from Alicyclobacillus acidocaldarius. Research Abstract Details 

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Heterologous expression of a gene encoding a thermostable beta-galactosidase from Alicyclobacillus acidocaldarius. Abstract Text:

Tiezheng Yuan,Peilong Yang,Yaru Wang,Kun Meng,Huiying Luo,Wei Zhang,Ningfeng Wu,Yunliu Fan,Bin Yao,

A genomic DNA library screen yielded the nucleotide sequence of a 12 kb fragment containing a gene (2067 bp) coding a thermostable beta-galactosidase from Alicyclobacillus acidocaldarius ATCC 27009. The beta-galactosidase gene was expressed in Pichia pastoris, and up to 90 mg recombinant beta-galactosidase/l accumulated in shake flask cultures. Using o-nitrophenyl-beta-D: -galactopyranoside as a substrate, the optimum pH and temperature of the purified recombinant beta-galactosidase were 5.8-6.0 and 70 degrees C, respectively. The enzyme retained 90% of its activity when heated at 70 degrees C for 30 min. Approximately 48% of lactose in milk was hydrolyzed following treatment with the recombinant enzyme over 60 min at 65 degrees C.

Heterologous expression of a gene encoding a thermostable beta-galactosidase from Alicyclobacillus acidocaldarius. Publishing Authors By Initials

T Yuan,P Yang,Y Wang,K Meng,H Luo,W Zhang,N Wu,Y Fan,B Yao,

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Heterologous expression of a gene encoding a thermostable beta-galactosidase from Alicyclobacillus acidocaldarius. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Biotechnology letters

VOLUME: 30

Page Numbers: 343-8

Journal Abbreviation: Biotechnol. Lett.

ISSN: 0141-5492

DAY: 4

MONTH: 10

YEAR: 2007

Heterologous expression of a gene encoding a thermostable beta-galactosidase from Alicyclobacillus acidocaldarius. Information

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LANGUAGE: eng

NlmUniqueID: 8008051

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Grant and Affiliation Information for Heterologous expression of a gene encoding a thermostable beta-galactosidase from Alicyclobacillus acidocaldarius.

AFFILIATION: Microbial Engineering Department, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, China, yaobin@caas-bio.net.cn.

Country: Netherlands

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MEDLINETA: Biotechnol Lett

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