Heterogeneity of methotrexate binding in human colon tumor cells.

Heterogeneity of methotrexate binding in human colon tumor cells. Research Abstract Details 

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Heterogeneity of methotrexate binding in human colon tumor cells. Abstract Text:

M P Iqbal,N Mehboobali,M A Waqar,M Ahmed,

[3H]-methotrexate binding at pH 5.0 and pH 7.2 by the cytosol of tumor tissues and the surrounding normal areas of the gastrointestinal tract of patients suffering from colon or gastric cancer has been used to identify in these cells the presence of a binder of methotrexate having low-affinity for this drug in addition to the enzyme dihydrofolate reductase. Scatchard analysis of the [3H]-methotrexate binding data by a colon tumor sample also reveals that there are two binders of this drug present in the cytosol of these cells. The association constant (Kass) for one binder of methotrexate is = 5.6 x 10(7) M-1 while the Kass for the second binder is = 1.0 x 10(6) M-1. The two binders do not differ very much in their apparent molecular weight. Upon isoelectric focusing, the tumor cell cytosol resolves into 4 major isoproteins each having the ability not only to bind [3H]-methotrexate but also reduce [3H]-pteroylglutamic acid to [3H]-tetrahydropteroylglutamic acid. This suggests that the two binders of methotrexate may be the two forms of dihydrofolate reductase having different affinities for this anticancer drug.

Heterogeneity of methotrexate binding in human colon tumor cells. Publishing Authors By Initials

MP Iqbal,N Mehboobali,MA Waqar,M Ahmed,

For similar complex mixtures: tissue extracts research abstracts see: complex mixtures: tissue extracts research

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Heterogeneity of methotrexate binding in human colon tumor cells. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: JPMA. The Journal of the Pakistan Medical Associat

VOLUME: 41

Page Numbers: 136-9

Journal Abbreviation:

ISSN: 0030-9982

DAY: 12

MONTH: Jun

YEAR: 1991

Heterogeneity of methotrexate binding in human colon tumor cells. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7501162

Heterogeneity of methotrexate binding in human colon tumor cells. Keywords Mesh Terms:

KEYWORDS: Tissue Extracts

MESH TERMS: metabolism

Chemical & Substance for Abstract: Heterogeneity of methotrexate binding in human colon tumor cells. Information

Substance Name: Tetrahydrofolate Dehydrogenase

Registry Number: EC 1.5.1.3

Grant and Affiliation Information for Heterogeneity of methotrexate binding in human colon tumor cells.

AFFILIATION: Department of Biochemistry, Aga Khan University, Karachi.

Country: PAKISTAN

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MEDLINETA: J Pak Med Assoc

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