Heterodimeric bone morphogenetic proteins show enhanced activity in vitro and in vivo.

Heterodimeric bone morphogenetic proteins show enhanced activity in vitro and in vivo. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Heterodimeric bone morphogenetic proteins show enhanced activity in vitro and in vivo. Abstract Text:

D I Israel,J Nove,K M Kerns,R J Kaufman,V Rosen,K A Cox,J M Wozney,

The bone morphogenetic proteins (BMPs), a subgroup of the TGF-beta gene super-family, are dimeric molecules involved in the growth, differentiation and repair of a wide variety of tissues. Based on the observation that several of the BMPs co-purify when isolated from bovine bone and that a pattern of co-localization exists during mouse embryogenesis, we co-expressed various combinations of BMPs in Chinese hamster ovary cells to test for possible heterodimer formation and activity. Transient co-expression of BMP-2 with either BMP-5, BMP-6 or BMP-7, or BMP-4 transiently co-expressed with BMP-7, resulted in more BMP activity than expression of any single BMP. Stable cell lines were then made in order to purify and characterize co-expressed BMPs in more detail. Co-expression of BMP-2 with BMP-7 yielded heterodimeric BMP-2/7 with a specific activity about 20-fold higher than BMP homodimers in an in vitro alkaline phosphatase induction assay. These heterodimers were also 5- to 10-fold more potent than BMP-2 in inducing cartilage and bone in an in vivo assay. Similar results were obtained with BMP-2/6 heterodimer. These experiments demonstrate the increased potency of several BMP heterodimers relative to BMP homodimers and support the hypothesis that such heterodimeric forms are likely to have natural biological functions.

Heterodimeric bone morphogenetic proteins show enhanced activity in vitro and in vivo. Publishing Authors By Initials

DI Israel,J Nove,KM Kerns,RJ Kaufman,V Rosen,KA Cox,JM Wozney,

For similar peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta research abstracts see: peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta research

PUBMED ID PMID:

MEDLINE DATE:

Heterodimeric bone morphogenetic proteins show enhanced activity in vitro and in vivo. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Growth factors (Chur, Switzerland)

VOLUME: 13

Page Numbers: 291-300

Journal Abbreviation: Growth Factors

ISSN: 0897-7194

DAY: 19

MONTH: 11

YEAR: 1996

Heterodimeric bone morphogenetic proteins show enhanced activity in vitro and in vivo. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9000468

Heterodimeric bone morphogenetic proteins show enhanced activity in vitro and in vivo. Keywords Mesh Terms:

KEYWORDS: Transforming Growth Factor beta

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Heterodimeric bone morphogenetic proteins show enhanced activity in vitro and in vivo. Information

Substance Name: Alkaline Phosphatase

Registry Number: EC 3.1.3.1

Grant and Affiliation Information for Heterodimeric bone morphogenetic proteins show enhanced activity in vitro and in vivo.

AFFILIATION: Genetics Institute, Cambridge, MA 02140, USA.

Country: SWITZERLAND

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Growth Factors

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Heterodimeric bone morphogenetic proteins show enhanced activity in vitro and in vivo Related Publications

• A single percutaneous injection of recombinant human bone morphogenetic protein-2 accelerates fracture repair.
• Biotinated bone morphogenetic protein-2: In vivo and in vitro activity.
• Characterization of rhBMP-2 pharmacokinetics implanted with biomaterial carriers in the rat ectopic model.
• Delivery systems for BMPs: factors contributing to protein retention at an application site.
• Engineering new bone with bone morphogenic proteins.
• Heterodimeric bone morphogenetic proteins show enhanced activity in vitro and in vivo.
• Mandibular reconstruction using bone morphogenetic protein 2: long-term follow-up in a canine model.
• Periodontal repair in dogs: effect of rhBMP-2 concentration on regeneration of alveolar bone and periodontal attachment.
• Periodontal repair in dogs: evaluation of a bioresorbable calcium phosphate cement (Ceredex) as a carrier for rhBMP-2.
• Recombinant human bone morphogenetic protein-2 accelerates healing in a rabbit ulnar osteotomy model.
• Recombinant human bone morphogenetic protein-2 and collagen for bone regeneration.
• RhBMP-2/alphaBSM induces significant vertical alveolar ridge augmentation and dental implant osseointegration.
• rhBMP-12 accelerates healing of rotator cuff repairs in a sheep model.