Hetero subunit interaction and RNA recognition of yeast tRNA (m7G46) methyltransferase synthesized in a wheat germ cell-free translation system.

Hetero subunit interaction and RNA recognition of yeast tRNA (m7G46) methyltransferase synthesized in a wheat germ cell-free translation system. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Hetero subunit interaction and RNA recognition of yeast tRNA (m7G46) methyltransferase synthesized in a wheat germ cell-free translation system. Abstract Text:

Yuki Muneyoshi,Keisuke Matsumoto,Chie Tomikawa,Takashi Toyooka,Anna Ochi,Takashi Masaoka,Yaeta Endo,Hiroyuki Hori,

Yeast tRNA (m(7)G46) methyltransferase contains two protein subunits (Trm8 and Trm82). The enzyme catalyzes a methyl-transfer from S-adenosyl-L-methionine to the N(7) atom of guanine at position 46 in tRNA. We deviced synthesis of active Trm8-Trm82 heterodimer in a wheat germ cell-free translation system. When Trm8 or Trm82 mRNA were used for a synthesis, Trm8 or Trm82 protein could be synthesized. Upon mixing the synthesized Trm8 and Trm82 proteins, no active Trm8-Trm82 heterodimer was produced. Active Trm8-Trm82 heterodimer was only synthesized under conditions, in which both Trm8 and Trm82 mRNAs were co-translated. To address the RNA recognition mechanism of the Trm8-Trm82 complex, we investigated methyl acceptance activities of eight truncated yeast tRNA(Phe) transcripts. In this meeting, we demonstrate that yeast Trm8-Trm82 has stricter recognition requirements for the tRNA molecule as compared to the bacterial enzyme, TrmB.

Hetero subunit interaction and RNA recognition of yeast tRNA (m7G46) methyltransferase synthesized in a wheat germ cell-free translation system. Publishing Authors By Initials

Y Muneyoshi,K Matsumoto,C Tomikawa,T Toyooka,A Ochi,T Masaoka,Y Endo,H Hori,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Hetero subunit interaction and RNA recognition of yeast tRNA (m7G46) methyltransferase synthesized in a wheat germ cell-free translation system. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Nucleic acids symposium series (2004)

VOLUME:

Page Numbers: 359-60

Journal Abbreviation: Nucleic Acids Symp Ser (Oxf)

ISSN: 1746-8272

DAY: 21

MONTH: 11

YEAR: 2007

Hetero subunit interaction and RNA recognition of yeast tRNA (m7G46) methyltransferase synthesized in a wheat germ cell-free translation system. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101259965

Hetero subunit interaction and RNA recognition of yeast tRNA (m7G46) methyltransferase synthesized in a wheat germ cell-free translation system. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Hetero subunit interaction and RNA recognition of yeast tRNA (m7G46) methyltransferase synthesized in a wheat germ cell-free translation system. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Hetero subunit interaction and RNA recognition of yeast tRNA (m7G46) methyltransferase synthesized in a wheat germ cell-free translation system.

AFFILIATION: Department of Materials and Biotechnology, Graduate School of Science and Engineering Ehime University, Matsuyama, 790-8577 Japan.

Country: England

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Nucleic Acids Symp Ser (Oxf)

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Hetero subunit interaction and RNA recognition of yeast tRNA m7G46 methyltransferase synthesized in a wheat germ cell-free translation system Related Publications

• A dibenzoylmethane derivative protects dopaminergic neurons against both oxidative stress and endoplasmic reticulum stress.
• A smart video magnifier controlled by the visibility signal of a low vision user.
• Adrenodoxin-cytochrome P450scc interaction as revealed by EPR spectroscopy: comparison with the putidaredoxin-cytochrome P450cam system.
• Compact synchrotron light source of the HSRC.
• Determination of nonylphenol ethoxylates and octylphenol ethoxylates in environmental samples using 13C-labeled surrogate compounds.
• Differences in substrate selectivities of the SPOUT superfamily of methyltransferases.
• Drug-resistance gene expression and progression of astrocytic tumors.
• Effect of combined humanPTH(1-34) and calcitonin treatment in ovariectomized rats.
• Effect of intracoronary thrombectomy on 30-day mortality in patients with acute myocardial infarction.
• Effect of molecular chirality and side chain bulkiness on angiogenesis of haloacetylcarbamoyl-2-nitroimidazole compounds.
• Effect on Outcome of an Increase of Serum Cardiac Troponin T in Patients With Healing or Healed ST-Elevation Myocardial Infarction.
• Enhancement of synaptic transmission and nociceptive behaviour in HPC-1/syntaxin 1A knockout mice following peripheral nerve injury.
• Hetero subunit interaction and RNA recognition of yeast tRNA (m7G46) methyltransferase synthesized in a wheat germ cell-free translation system.
• Hydroxyl radical probing of tRNA (Gm18) methyltransferase [TrmH]-AdoMet-artificial tRNA ternary complex.
• Influence of the signal-to-noise ratio on the accuracy of IOLMaster measurements.
• Isolation of diacylglycerol-2-aminoethylphosphonate from Tetrahymena pyriformis.
• Long-term oral administration of dipyridamole improves both cardiac and physical status in patients with mild to moderate chronic heart failure: a prospective open-randomized study.
• Modified permanent middle cerebral artery occlusion rat model aiming to reduce variability in infarct size.
• Motor evoked potential mapping and monitoring by direct brainstem stimulation. Technical note.
• Nicardipine prolonged-release implants for preventing cerebral vasospasm after subarachnoid hemorrhage: effect and outcome in the first 100 patients.
• Perioperative plasma active and total ghrelin levels are reduced in acromegaly when compared with in nonfunctioning pituitary tumours even after normalization of serum GH.
• Rho-kinase activation in endothelial cells contributes to expansion of infarction after focal cerebral ischemia.
• Right-handed snakes: convergent evolution of asymmetry for functional specialization.
• Selective peripheral denervation of the levator scapulae muscle for laterocollic cervical dystonia.
• Sequence motif-specific assignment of two [2Fe-2S] clusters in rat xanthine oxidoreductase studied by site-directed mutagenesis.
• The carboxyl-terminus directs TAF(I)48 to the nucleus and nucleolus and associates with multiple nuclear import receptors.
• Vertebrate collagenase: direct extraction from animal skin and human synovial membrane.
• Visual emotional context modulates brain potentials elicited by unattended tones.
• [Current status and problems in approval of new anticancer drugs in Japan]
• [Jervell and Lange-Nielsen syndrome]