HES6 reverses nuclear reprogramming of insulin-producing cells following cell fusion.

HES6 reverses nuclear reprogramming of insulin-producing cells following cell fusion. Research Abstract Details 

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HES6 reverses nuclear reprogramming of insulin-producing cells following cell fusion. Abstract Text:

Andrew J Ball,Annelie E Abrahamsson, Tyrberg,Pamela Itkin-Ansari,Fred Levine,

To examine the mechanism by which growth-stimulated pancreatic beta-cells dedifferentiate, somatic cell fusions were performed between MIN6, a highly differentiated mouse insulinoma, and betalox5, a cell line derived from human beta-cells which progressively dedifferentiated in culture. MIN6/betalox5 somatic cells hybrids underwent silencing of insulin expression and a marked decline in PDX1, NeuroD, and MafA, indicating that betalox5 expresses a dominant transacting factor(s) that represses beta-cell differentiation. Expression of Hes1, which inhibits endocrine differentiation was higher in hybrid cells than in parental MIN6 cells. Hes6, a repressor of Hes1, was highly expressed in primary beta-cells as well as MIN6, but was repressed in hybrids. Hes6 overexpression using a retroviral vector led to a decrease in Hes1 levels, an increase in beta-cell transcription factors and partial restoration of insulin expression. We conclude that the balance of Notch activators and inhibitors may play an important role in maintaining the beta-cell differentiated state.

HES6 reverses nuclear reprogramming of insulin-producing cells following cell fusion. Publishing Authors By Initials

AJ Ball,AE Abrahamsson,B Tyrberg,P Itkin-Ansari,F Levine,

For similar proteins: dna-binding proteins: trans-activators research abstracts see: proteins: dna-binding proteins: trans-activators research

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HES6 reverses nuclear reprogramming of insulin-producing cells following cell fusion. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Biochemical and biophysical research communication

VOLUME: 355

Page Numbers: 331-7

Journal Abbreviation: Biochem. Biophys. Res. Commun.

ISSN: 0006-291X

DAY: 5

MONTH: 02

YEAR: 2007

HES6 reverses nuclear reprogramming of insulin-producing cells following cell fusion. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 372516

HES6 reverses nuclear reprogramming of insulin-producing cells following cell fusion. Keywords Mesh Terms:

KEYWORDS: Trans-Activators

MESH TERMS: metabolism

Chemical & Substance for Abstract: HES6 reverses nuclear reprogramming of insulin-producing cells following cell fusion. Information

Substance Name: Insulin

Registry Number: 11061-68-0

Grant and Affiliation Information for HES6 reverses nuclear reprogramming of insulin-producing cells following cell fusion.

AFFILIATION: UCSD Department of Pediatrics, University of California-San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0816, USA.

Country: United States

AGENCY: United States NIDDK

GRANT: U19 DK061248-03S2000

ACRONYM: DK

MEDLINETA: Biochem Biophys Res Commun

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