HEPES-stabilized encapsulation of Salmonella typhimurium.

HEPES-stabilized encapsulation of Salmonella typhimurium. Research Abstract Details 

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HEPES-stabilized encapsulation of Salmonella typhimurium. Abstract Text:

Zhiyong Suo,Xinghong Yang,Recep Avci,Laura Kellerman,David W Pascual,Marc Fries,Andrew Steele,

Most bacteria, planktonic and sessile, are encapsulated inside loosely bound extracellular polymeric substance (EPS) in their physiological environment. Imaging a bacterium with its capsule requires lengthy sample preparation to enhance the capsular contrast. In this study, Salmonella typhimurium was investigated using atomic force microscopy for a practical means of imaging an encapsulated bacterium in air. The investigation further aimed to determine the relation between the buffers used for preparing the bacterium and the preservation of the capsular material surrounding it. It was observed that rinsing bacteria with HEPES buffer could stabilize and promote capsule formation, while rinsing with PBS, Tris, or glycine removes most of the capsular EPS. For bacteria rinsed with HEPES and air-dried, the height images showed only the contour of the capsular material, while the phase and amplitude images presented the detailed structures of the bacterial surface, including the flagella encapsulated inside the capsular EPS. The encapsulation was attributed to the cross-linking of the acidic exopolysaccharides mediated by the piperazine moiety of HEPES through electrostatic attraction. This explanation is supported by encapsulated bacteria observed for samples rinsed with N,N'-bis(2-hydroxyethyl)-piperazine solution and by the presence of entrapped HEPES within the dry capsular EPS suggested by micro-Raman spectroscopy.

HEPES-stabilized encapsulation of Salmonella typhimurium. Publishing Authors By Initials

Z Suo,X Yang,R Avci,L Kellerman,DW Pascual,M Fries,A Steele,

For similar bacteria: gram-negative bacteria: gram-negative facultatively anaerobic rods: enterobacteriaceae: salmonella: salmonella enterica: salmonella typhimurium research abstracts see: bacteria: gram-negative bacteria: gram-negative facultatively anaerobic rods: enterobacteriaceae: salmonella: salmonella enterica: salmonella typhimurium research

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HEPES-stabilized encapsulation of Salmonella typhimurium. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Langmuir : the ACS journal of surfaces and colloid

VOLUME: 23

Page Numbers: 1365-74

Journal Abbreviation:

ISSN: 0743-7463

DAY: 30

MONTH: Jan

YEAR: 2007

HEPES-stabilized encapsulation of Salmonella typhimurium. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9882736

HEPES-stabilized encapsulation of Salmonella typhimurium. Keywords Mesh Terms:

KEYWORDS: Salmonella typhimurium

MESH TERMS: metabolism

Chemical & Substance for Abstract: HEPES-stabilized encapsulation of Salmonella typhimurium. Information

Substance Name: HEPES

Registry Number: 7365-45-9

Grant and Affiliation Information for HEPES-stabilized encapsulation of Salmonella typhimurium.

AFFILIATION: Imaging and Chemical Analysis Laboratory, Department of Physics, Montana State University, Bozeman, MT 59717, USA.

Country: United States

AGENCY: United States NIAID

GRANT: AI 41123

ACRONYM: AI

MEDLINETA: Langmuir

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