Hepatocyte nuclear factor-4alpha and -1 small interfering RNA inhibits hepatocyte differentiation induced by extracellular matrix.

Hepatocyte nuclear factor-4alpha and -1 small interfering RNA inhibits hepatocyte differentiation induced by extracellular matrix. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Hepatocyte nuclear factor-4alpha and -1 small interfering RNA inhibits hepatocyte differentiation induced by extracellular matrix. Abstract Text:

Takayuki Kimata,Masahito Nagaki,Yoshihiko Tsukada,Tomio Ogiso,Hisataka Moriwaki,

Liver-specific genes and hepatocyte nuclear factor (HNF)-4alpha and -1 are coordinately regulated by extracellular matrix (ECM). However, still are unclear interactions between liver-specific genes and these liver-enriched transcription factors in the mechanism of hepatocyte differentiation regulated by ECM. To elucidate the relationship, we used small interfering RNA (siRNA), which obtains strong and specific knockdown of gene expression in cell culture. Treatment with siHNF-4alpha and siHNF-1 declined the expression levels for HNF-4alpha mRNA and HNF-1 mRNA in primary rat hepatocytes, respectively. The mRNA expressions of albumin, transthyretin, and apolipoproteins that were up-regulated in hepatocytes cultured on a basement membrane matrix, Engelbreth-Holm-Swarm (EHS) gel, were decreased in the presence of siHNF-4alpha or siHNF-1. Moreover, siHNF-4alpha and siHNF-1 did not affect the morphology and actin assembly of hepatocytes. These findings demonstrated that HNF-4alpha and HNF-1 directly regulate liver-specific gene expression and might be downstream of cytoskeletal organization in the mechanism by which the differentiated phenotype of hepatocytes is regulated by EHS gel.

Hepatocyte nuclear factor-4alpha and -1 small interfering RNA inhibits hepatocyte differentiation induced by extracellular matrix. Publishing Authors By Initials

T Kimata,M Nagaki,Y Tsukada,T Ogiso,H Moriwaki,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Hepatocyte nuclear factor-4alpha and -1 small interfering RNA inhibits hepatocyte differentiation induced by extracellular matrix. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Hepatology research : the official journal of the

VOLUME: 35

Page Numbers: 3-9

Journal Abbreviation:

ISSN: 1386-6346

DAY: 24

MONTH: 03

YEAR: 2006

Hepatocyte nuclear factor-4alpha and -1 small interfering RNA inhibits hepatocyte differentiation induced by extracellular matrix. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9711801

Hepatocyte nuclear factor-4alpha and -1 small interfering RNA inhibits hepatocyte differentiation induced by extracellular matrix. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Hepatocyte nuclear factor-4alpha and -1 small interfering RNA inhibits hepatocyte differentiation induced by extracellular matrix. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Hepatocyte nuclear factor-4alpha and -1 small interfering RNA inhibits hepatocyte differentiation induced by extracellular matrix.

AFFILIATION: Department of Gastroenterology, Gifu University Graduate School of Medicine, Gifu 501-1194, Japan.

Country: Netherlands

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Hepatol Res

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Hepatocyte nuclear factor-4alpha and -1 small interfering RNA inhibits hepatocyte differentiation induced by extracellular matrix Related Publications

• Atypical eosinophilic angiocentric fibrosis on nasal septum.
• Biochemical studies on kallikreins and their related substances. II. An improved method of purification of hog pancreatic kallikreinand its some biological properties.
• Continuous drip infusion of low dose cytarabine and etoposide with granulocyte colony-stimulating factor for elderly patients with acute myeloid leukaemia ineligible for intensive chemotherapy.
• Detection of arginine esterase of various kallikrein preparations on gellified electrophoretic media.
• Effect of enzymatically modified isoquercitrin in spontaneously hypertensive rats.
• Expression of GM1 and GD1a in liver of wild mice.
• Expression of GM1 and GD1a in mouse liver is linked to the H-2 complex on chromosome 17.
• Expression of retinoid X receptor alpha is decreased in 3'-methyl-4-dimethylaminoazobenzene-induced hepatocellular carcinoma in rats.
• Granulocyte colony-stimulating factor impairs liver regeneration in mice through the up-regulation of interleukin-1beta.
• Hepatocyte nuclear factor-4alpha and -1 small interfering RNA inhibits hepatocyte differentiation induced by extracellular matrix.
• Hepatoprotection against oxidative damage.
• Histological evaluation of radiochemotherapy for prostatic cancer: early results of a pilot study.
• Immunoproliferative Small Intestinal Disease with Protein Loss Complicated with Duodenal T Cell Lymphoma during Progression.
• Inhibition of nuclear factor-kappa B induces inflammatory cell migration and exacerbates severe liver injury in hepatitis B virus transgenic mice.
• Liver injury induced by lipopolysaccharide is mediated by TNFR-1 but not by TNFR-2 or Fas in mice.
• Oral branched-chain amino acid supplementation improves the oxidized/reduced albumin ratio in patients with liver cirrhosis.
• Phosphorylated retinoid X receptor alpha loses its heterodimeric activity with retinoic acid receptor beta.
• Prevalence of diabetes and incidence of angiopathy in patients with chronic viral liver disease.
• Prospective study on early virologic response to treatment with interferon alpha-2b plus ribavirin in patients with chronic hepatitis C genotype 1b.
• Purification of cat submaxillary kallikrein.
• Relationship between serum albumin level and aging in community-dwelling self-supported elderly population.
• Studies of kallikrein inhibitors in potatoes. I. Partial purification and some properties.
• Studies of kallikrein inhibitors in potatoes. II. Effect of potato kallikrein inhibitors on various kallikreins and other proteases.
• Studies on human salivary kallikrein. I. Isolation of human salivary kallikrein.
• Studies on urinary kallikreins. I. Purification and characterization of human urinary kallikreins.
• Synergistic effects of RXR alpha and PPAR gamma ligands to inhibit growth in human colon cancer cells--phosphorylated RXR alpha is a critical target for colon cancer management.
• Thick-section half-Fourier rapid acquisition with relaxation enhancement MR cholangiopancreatography: effects of i.v. administration of gadolinium chelate.
• Vibration transducer using an optical cavity comprising birefringent mirrors.
• [Personality: Ms. Yuko Moriwaki, a free-lance journalist who received an award for an article on the welfare of the aged]
• [Significance and methods of reassignment of nursing supervisors]