Hepatitis C virus genotype 1b chimeric replicon containing genotype 3 NS5A domain.

Hepatitis C virus genotype 1b chimeric replicon containing genotype 3 NS5A domain. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Hepatitis C virus genotype 1b chimeric replicon containing genotype 3 NS5A domain. Abstract Text:

Robert E Lanford,Bernadette Guerra,Helen Lee,

Infections with hepatitis C virus (HCV) genotype 3 exhibit differences in clinical phenotype including an increase in response to interferon therapy and development of steatosis. To initiate studies on genotype 3, we created a chimeric genotype 1b replicon containing a genotype 3a NS5A domain. The chimera was capable of efficient colony formation after the selection of a novel dominant adaptive mutation. Thus, domains from highly different strains can interact to form a functional replicase. A new genotype 1a replicon was constructed as well. Genotype specific influence on interferon sensitivity was examined using genotype 1a, 1b and chimeric 1b-3a replicons. The genotype 3a NS5A domain did not increase the sensitivity of the chimeric replicon to IFNalpha. The results suggest that NS5A is not sufficient to convey the increased IFNalpha response by genotype 3 or the replicon model is not capable of mimicking the events involved in increased sustained viral response.

Hepatitis C virus genotype 1b chimeric replicon containing genotype 3 NS5A domain. Publishing Authors By Initials

RE Lanford,B Guerra,H Lee,

For similar biological phenomena, cell phenomena, and immunity: biological phenomena: microbiologic phenomena: viral physiology: virus replication research abstracts see: biological phenomena, cell phenomena, and immunity: biological phenomena: microbiologic phenomena: viral physiology: virus replication research

PUBMED ID PMID:

MEDLINE DATE:

Hepatitis C virus genotype 1b chimeric replicon containing genotype 3 NS5A domain. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Virology

VOLUME: 355

Page Numbers: 192-202

Journal Abbreviation: Virology

ISSN: 0042-6822

DAY: 21

MONTH: 08

YEAR: 2006

Hepatitis C virus genotype 1b chimeric replicon containing genotype 3 NS5A domain. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 110674

Hepatitis C virus genotype 1b chimeric replicon containing genotype 3 NS5A domain. Keywords Mesh Terms:

KEYWORDS: Virus Replication

MESH TERMS: drug effects

Chemical & Substance for Abstract: Hepatitis C virus genotype 1b chimeric replicon containing genotype 3 NS5A domain. Information

Substance Name: Viral Nonstructural Proteins

Registry Number: 0

Grant and Affiliation Information for Hepatitis C virus genotype 1b chimeric replicon containing genotype 3 NS5A domain.

AFFILIATION: Department of Virology and Immunology, Southwest National Primate Research Center, Southwest Foundation for Biomedical Research, 7620 NW Loop 410, San Antonio, TX 78227, USA. rlanford@icarus.sfbr.org

Country: United States

AGENCY: United States NIAID

GRANT: U19 AI40035

ACRONYM: AI

MEDLINETA: Virology

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Hepatitis C virus genotype 1b chimeric replicon containing genotype 3 NS5A domain Related Publications

• A differential response of two putative mammalian circadian regulators, mper1 and mper2, to light.
• Actual Conditions and Countermeasure of Yun-bian Old Men's Health.
• Aspirin changes the secretion rate and amino acid composition of human small intestinal mucin in subjects with ileal conduits.
• Bone morphogenetic protein-2 facilitates expression of chondrogenic, not osteogenic, phenotype of human intervertebral disc cells.
• Channel architecture in maltoporin: dominance studies with lamB mutations influencing maltodextrin binding provide evidence for independent selectivity filters in each subunit.
• Compensation. Costly to a fault.
• Design and feasibility of quick EXAFS scans for a 'broomstick' double-crystal monochromator at PLS beamline.
• Directed evolution of the lambda receptor of Escherichia coli through affinity chromatographic selection.
• Effects of dietary vitamin E supplementation on lipid oxidation and volatiles content of irradiated, cooked turkey meat patties with different packaging.
• Exclusion of high-molecular-weight maltosaccharides by lipopolysaccharide O-antigen of Escherichia coli and Salmonella typhimurium.
• Family decision making for elderly patients in the intensive care unit.
• Identification of an osteogenic protein-1 responsive element in the aggrecan promoter.
• Impairment of conjunctival glutathione secretion and ion transport by oxidative stress in an adenovirus type 5 ocular infection model of pigmented rabbits.
• Inactivation of the PTEN gene protein product is associated with the invasiveness and metastasis, but not angiogenesis, of breast cancer.
• Isolation, by affinity chromatography, of mutant escherichia coli cells with novel regulation of lamB expression.
• Longitudinal clivus fracture associated with trapped basilar artery: unusual survival with good neurological recovery.
• MPer1 and mper2 are essential for normal resetting of the circadian clock.
• Model construction on quality of family caregiving in the elderly.
• Nonredundant roles of the mPer1 and mPer2 genes in the mammalian circadian clock.
• Photoionization studies of sulfur radicals and products of their reactions.
• Posterior transarticular screw fixation for chronic atlanto-axial instability.
• Reciprocal regulation of haem biosynthesis and the circadian clock in mammals.
• Restoration of a loose implant supported crown: a case report.
• Significance of chronic epilepsy in glial tumors and correlation with surgical strategies.
• The circadian gene Period2 plays an important role in tumor suppression and DNA damage response in vivo.
• The effects of permeation enhancers on the surface morphology of the rat nasal mucosa: a scanning electron microscopy study.
• The influence of maltoporin affinity on the transport of maltose and maltohexaose into Escherichia coli.
• Tissue-specific expression, heat inducibility, and biological roles of two hsp16 genes in Caenorhabditis elegans.
• Up-regulation of MUC5AC and MUC5B mucin genes in chronic rhinosinusitis.
• [Expression of MUC3, MUC5AC, MUC6 and epidermal growth factor receptor in gallbladder epithelium according to gallstone composition]