Hepatic transcript levels for genes coding for enzymes associated with xenobiotic metabolism are altered with age.

Hepatic transcript levels for genes coding for enzymes associated with xenobiotic metabolism are altered with age. Research Abstract Details 

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Hepatic transcript levels for genes coding for enzymes associated with xenobiotic metabolism are altered with age. Abstract Text:

Kazuhiko Mori,Pamela E Blackshear,Edward K Lobenhofer,Joel S Parker,Denise P Orzech,Joseph H Roycroft,Kimwa L Walker,Kennita A Johnson,Tiwanda A Marsh,Richard D Irwin,Gary A Boorman,

Metabolism studies are crucial for data interpretation from rodent toxicity and carcinogenicity studies. Metabolism studies are usually conducted in 6 to 8 week old rodents. Long-term studies often continue beyond 100 weeks of age. The potential for age-related changes in transcript levels of genes encoding for enzymes associated with metabolism was evaluated in the liver of male F344/N rats at 32, 58, and 84 weeks of age. Differential expression was found between the young and old rats for genes whose products are involved in both phase I and phase II metabolic pathways. Thirteen cytochrome P450 genes from CYP families 1-3 showed alterations in expression in the older rats. A marked age-related decrease in expression was found for 4 members of the Cyp3a family that are critical for drug metabolism in the rat. Immunohistochemical results confirmed a significant decrease in Cyp3a2 and Cyp2c11 protein levels with age. This indicates that the metabolic capacity of male rats changes throughout a long-term study. Conducting multiple hepatic microarray analyses during the conduct of a long-term study can provide a global view of potential metabolic changes that might occur. Alterations that are considered crucial to the interpretation of long-term study results could then be confirmed by subsequent metabolic studies.

Hepatic transcript levels for genes coding for enzymes associated with xenobiotic metabolism are altered with age. Publishing Authors By Initials

K Mori,PE Blackshear,EK Lobenhofer,JS Parker,DP Orzech,JH Roycroft,KL Walker,KA Johnson,TA Marsh,RD Irwin,GA Boorman,

For similar pharmaceutical preparations: xenobiotics research abstracts see: pharmaceutical preparations: xenobiotics research

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Hepatic transcript levels for genes coding for enzymes associated with xenobiotic metabolism are altered with age. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Intr

Journal: Toxicologic pathology

VOLUME: 35

Page Numbers: 242-51

Journal Abbreviation:

ISSN: 0192-6233

DAY: 3

MONTH: 12

YEAR: 2007

Hepatic transcript levels for genes coding for enzymes associated with xenobiotic metabolism are altered with age. Information

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LANGUAGE: eng

NlmUniqueID: 7905907

Hepatic transcript levels for genes coding for enzymes associated with xenobiotic metabolism are altered with age. Keywords Mesh Terms:

KEYWORDS: Xenobiotics

MESH TERMS: metabolism

Chemical & Substance for Abstract: Hepatic transcript levels for genes coding for enzymes associated with xenobiotic metabolism are altered with age. Information

Substance Name: Steroid 16-alpha-Hydroxylase

Registry Number: EC 1.14.14.1

Grant and Affiliation Information for Hepatic transcript levels for genes coding for enzymes associated with xenobiotic metabolism are altered with age.

AFFILIATION: National Institute of Environmental Health Sciences, Research Triangle Park, NC 27701, USA.

Country: United States

AGENCY: United States NIEHS

GRANT: N01-ES-25497

ACRONYM: ES

MEDLINETA: Toxicol Pathol

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