Heparan sulfate proteoglycans mediate Staphylococcus aureus interactions with intestinal epithelium.

Heparan sulfate proteoglycans mediate Staphylococcus aureus interactions with intestinal epithelium. Research Abstract Details 

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Heparan sulfate proteoglycans mediate Staphylococcus aureus interactions with intestinal epithelium. Abstract Text:

Donavon J Hess,Michelle J Henry-Stanley,Stanley L Erlandsen,Carol L Wells,

Staphylococcus aureus can be internalized by non-professional phagocytes, and may colonize the intestine in normal and antibiotic-treated individuals. Intestinal colonization may depend on the interactions of S. aureus with the intestinal epithelium. The best described mechanism of S. aureus binding to eukaryotic cells involves S. aureus fibronectin binding proteins (FnBPs), using fibronectin as a bridging molecule to beta1 integrins on the eukaryotic cell surface. Because S. aureus can be internalized by enterocytes, and because S. aureus is known to bind heparan sulfate (HS), we hypothesized that heparan sulfate proteoglycans (HSPGs) widely expressed on epithelia may mediate S. aureus interactions with intestinal epithelial cells. Internalization of S. aureus RN6390 by cultured intestinal epithelial cells was inhibited in a dose-dependent fashion by the HS mimic heparin, and by HS itself. Internalization of S. aureus DU5883, which lacks expression of staphylococcal FnBPs, was also inhibited by heparin. S. aureus adherence to ARH-77 cells, transfected to express the HSPG syndecan-1, was greatly increased when compared to adherence to plasmid control ARH-77 cells which have little detergent extractable HS. In addition, compared to wild-type HS-expressing Chinese hamster ovary (CHO) cells, internalization of S. aureus was decreased using mutant CHO cells with decreased HS expression. These findings are consistent with a model wherein S. aureus internalization by intestinal epithelial cells (and perhaps other epithelia) is mediated by S. aureus binding to the HS moiety of cell-surface HSPGs, and this interaction appears independent of fibronectin binding.

Heparan sulfate proteoglycans mediate Staphylococcus aureus interactions with intestinal epithelium. Publishing Authors By Initials

DJ Hess,MJ Henry-Stanley,SL Erlandsen,CL Wells,

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Heparan sulfate proteoglycans mediate Staphylococcus aureus interactions with intestinal epithelium. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Medical microbiology and immunology

VOLUME: 195

Page Numbers: 133-41

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ISSN: 0300-8584

DAY: 24

MONTH: 12

YEAR: 2005

Heparan sulfate proteoglycans mediate Staphylococcus aureus interactions with intestinal epithelium. Information

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LANGUAGE: eng

NlmUniqueID: 314524

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Grant and Affiliation Information for Heparan sulfate proteoglycans mediate Staphylococcus aureus interactions with intestinal epithelium.

AFFILIATION: Department of Surgery, University of Minnesota, Minneapolis, MN 55455, USA.

Country: Germany

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MEDLINETA: Med Microbiol Immunol

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