Hemocyanin from Tachypleus gigas. II. Cooperative interactions of the subunits.

Hemocyanin from Tachypleus gigas. II. Cooperative interactions of the subunits. Research Abstract Details 

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Hemocyanin from Tachypleus gigas. II. Cooperative interactions of the subunits. Abstract Text:

N Makino,

Six subunits (I to VI) were isolated from hemocyanin of an Asian horseshoe crab, Tachypleus gigas, by anion exchange chromatography of the dissociated hemocyanin. The subunit preparations were nearly homogeneous as judged by alkaline electrophoresis, but they still showed the presence of isoproteins in isoelectric focusing. The subunits were reassembled (in 10 mM CaCl2 at pH 7.5) and tested for restoration of the cooperativity in O2 binding. The reassembly of the subunits gave equilibrium mixtures of the monomer and hexamer with small amounts of larger molecules. Homogeneous and heterogeneous hexamers were prepared by reassembling a single kind or two kinds of subunits, followed by isolation of the hexamer fraction by gel filtration. Among the homohexamers, only the subunit V hexamer showed cooperativity in O2 binding with the Hill coefficient of 1.6. Among the heterohexamers the subunit I/V hybrid was most noteworthy, showing a Hill coefficient (1.7) higher than that of any other heterohexamer examined. It was concluded that there are specific interactions between the subunits I and V. It is suggested that their interactions are important for the cooperativity in the native hemocyanin.

Hemocyanin from Tachypleus gigas. II. Cooperative interactions of the subunits. Publishing Authors By Initials

N Makino,

For similar biochemical phenomena, metabolism, and nutrition: biochemical phenomena: protein binding research abstracts see: biochemical phenomena, metabolism, and nutrition: biochemical phenomena: protein binding research

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Hemocyanin from Tachypleus gigas. II. Cooperative interactions of the subunits. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 106

Page Numbers: 423-9

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Sep

YEAR: 1989

Hemocyanin from Tachypleus gigas. II. Cooperative interactions of the subunits. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

Hemocyanin from Tachypleus gigas. II. Cooperative interactions of the subunits. Keywords Mesh Terms:

KEYWORDS: Protein Binding

MESH TERMS: physiology

Chemical & Substance for Abstract: Hemocyanin from Tachypleus gigas. II. Cooperative interactions of the subunits. Information

Substance Name: Hemocyanin

Registry Number: 9013-72-3

Grant and Affiliation Information for Hemocyanin from Tachypleus gigas. II. Cooperative interactions of the subunits.

AFFILIATION: Division of Biochemistry, University of Tsukuba.

Country: JAPAN

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MEDLINETA: J Biochem

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