Helper-dependent adenovirus-mediated gene transfer into the adult mouse cochlea.

Helper-dependent adenovirus-mediated gene transfer into the adult mouse cochlea. Research Abstract Details 

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Helper-dependent adenovirus-mediated gene transfer into the adult mouse cochlea. Abstract Text:

Gentiana I Wenzel,Anping Xia,Etai Funk,M Bradley Evans,Donna J Palmer,Philip Ng,Fred A Pereira,John S Oghalai,Gentiana I Wenzel,Anping Xia,Etai Funk,M Bradley Evans,Donna J Palmer,Philip Ng,Fred A Pereira,John S Oghalai,Gentiana I Wenzel,Anping Xia,Etai Funk,M Bradley Evans,Donna J Palmer,Philip Ng,Fred A Pereira,John S Oghalai,

BACKGROUND: Gene therapy may provide a way to restore cochlear function to deaf patients. The most successful techniques for cochlear gene therapy have been injection of early-generation adenoviral vectors into scala media in guinea pigs. However, it is important to be able to perform gene therapy research in mice because there is wide availability of transgenic strains with hereditary hearing loss. PURPOSE: We demonstrate our technique for delivery of a third-generation adenoviral vector, helper-dependent adenovirus (HDAd), to the adult mouse cochlea. METHODS: Mice were injected with an HDAd that contained a reporter gene for either beta-galactosidase or green fluorescent protein into scala media. After 4 days, the cochleae were harvested for analyses. Auditory brainstem response monitoring of cochlear function was performed before making a cochleostomy, after making a cochleostomy, and before killing the animal. RESULTS: Beta-galactosidase was identified in the spiral ligament, the organ of Corti, and spiral ganglion cells by light microscopy. Green fluorescent protein epifluorescence was assessed in whole-mount organ of Corti preparations using confocal microscopy. This demonstrated transduction of inner hair cells, outer hair cells, and supporting cells. Paraffin-embedded cross sections similarly revealed gene transduction within the organ of Corti. Threshold shifts of 39.8 +/- 5.4 and 37.7 +/- 5.5 dB were observed in mice injected with HDAd or control buffer, respectively. CONCLUSION: The technique of scala media HDAd injection reliably infects the adult mouse cochlea, including cells within the organ of Corti, although the procedure itself adversely affects hearing.

Helper-dependent adenovirus-mediated gene transfer into the adult mouse cochlea. Publishing Authors By Initials

GI Wenzel,A Xia,E Funk,MB Evans,DJ Palmer,P Ng,FA Pereira,JS Oghalai,GI Wenzel,A Xia,E Funk,MB Evans,DJ Palmer,P Ng,FA Pereira,JS Oghalai,GI Wenzel,A Xia,E Funk,MB Evans,DJ Palmer,P Ng,FA Pereira,JS Oghalai,

For similar enzymes and coenzymes: enzymes: hydrolases: glycoside hydrolases: galactosidases: beta-galactosidase research abstracts see: enzymes and coenzymes: enzymes: hydrolases: glycoside hydrolases: galactosidases: beta-galactosidase research

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Helper-dependent adenovirus-mediated gene transfer into the adult mouse cochlea. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Otology & neurotology : official publication of th

VOLUME: 28

Page Numbers: 1100-8

Journal Abbreviation: Otol. Neurotol.

ISSN: 1531-7129

DAY: 8

MONTH: Dec

YEAR: 2007

Helper-dependent adenovirus-mediated gene transfer into the adult mouse cochlea. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 100961504

Helper-dependent adenovirus-mediated gene transfer into the adult mouse cochlea. Keywords Mesh Terms:

KEYWORDS: beta-Galactosidase

MESH TERMS: genetics

Chemical & Substance for Abstract: Helper-dependent adenovirus-mediated gene transfer into the adult mouse cochlea. Information

Substance Name: beta-Galactosidase

Registry Number: EC 3.2.1.23

Grant and Affiliation Information for Helper-dependent adenovirus-mediated gene transfer into the adult mouse cochlea.

AFFILIATION: Bobby R. Alford Department of Otolaryngology-Head and Neck Surgery, Baylor College of Medicine, Houston, Texas 77030, USA.

Country: United States

AGENCY: United States PHS

GRANT: R01-067324

ACRONYM: DC

MEDLINETA: Otol Neurotol

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