HeLa cell entry by guanidinium-rich beta-peptides: importance of specific cation-cell surface interactions.

HeLa cell entry by guanidinium-rich beta-peptides: importance of specific cation-cell surface interactions. Research Abstract Details 

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HeLa cell entry by guanidinium-rich beta-peptides: importance of specific cation-cell surface interactions. Abstract Text:

Terra B Potocky,John Silvius,Anant K Menon,Samuel H Gellman,

Short cationic oligomers, including arginine-rich peptides and analogous beta-amino acid oligomers ("beta-peptides"), can enter the cytoplasm and nucleus of a living cell from the extracellular medium. It seems increasingly clear that multiple entry pathways are possible, depending upon the structure of the guanidinium-rich molecule, the type of cell, and other factors. We have previously shown that conformational stability and spatial clustering of guanidinium groups increase the HeLa cell entry efficiency of short helical beta-peptides bearing six guanidinium groups, results that suggest that these beta-peptides could be useful tools for studying the entry process. Here we describe studies intended to identify the point in the entry process at which helix stability and spatial arrangement of guanidinium groups exert their effect. Our results suggest that key distinctions involve the mode of interaction between different guanidinium-rich beta-peptides and the HeLa cell surface. A specific guanidinium display appears to be required for proper engagement of cell-surface heparan sulfate proteoglycans and concomitant induction of endocytic uptake.

HeLa cell entry by guanidinium-rich beta-peptides: importance of specific cation-cell surface interactions. Publishing Authors By Initials

TB Potocky,J Silvius,AK Menon,SH Gellman,

For similar natural sciences: time: time factors research abstracts see: natural sciences: time: time factors research

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HeLa cell entry by guanidinium-rich beta-peptides: importance of specific cation-cell surface interactions. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Chembiochem : a European journal of chemical biolo

VOLUME: 8

Page Numbers: 917-26

Journal Abbreviation: Chembiochem

ISSN: 1439-4227

DAY: 25

MONTH: May

YEAR: 2007

HeLa cell entry by guanidinium-rich beta-peptides: importance of specific cation-cell surface interactions. Information

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LANGUAGE: eng

NlmUniqueID: 100937360

HeLa cell entry by guanidinium-rich beta-peptides: importance of specific cation-cell surface interactions. Keywords Mesh Terms:

KEYWORDS: Time Factors

MESH TERMS: pharmacology

Chemical & Substance for Abstract: HeLa cell entry by guanidinium-rich beta-peptides: importance of specific cation-cell surface interactions. Information

Substance Name: Epidermal Growth Factor

Registry Number: 62229-50-9

Grant and Affiliation Information for HeLa cell entry by guanidinium-rich beta-peptides: importance of specific cation-cell surface interactions.

AFFILIATION: Department of Chemistry, University of Wisconsin, Madison, WI 53706, USA.

Country: Germany

AGENCY: United States NIGMS

GRANT: GM56414

ACRONYM: GM

MEDLINETA: Chembiochem

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