Heat stress facilitates stretch-induced hypertrophy of cultured muscle cells.

Heat stress facilitates stretch-induced hypertrophy of cultured muscle cells. Research Abstract Details 

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Heat stress facilitates stretch-induced hypertrophy of cultured muscle cells. Abstract Text:

Katsumasa Yamashita-Goto,Yoshinobu Ohira,Ryoko Okuyama,Hitoshi Sugiyama,Masanori Honda,Takao Sugiura,Shigeru Yamada,Tatsuo Akema,Toshitada Yoshioka,

Increased mechanical stress induced by stretch is an important growth stimulus in skeletal muscle. Heat shock proteins (HSPs) are an important family of endogenous, protective proteins. HSP90 and HSP70 families show elevated levels under beat stress. Mechanical stress, such as physical exercise, is known to induce not only muscular hypertrophy but also the elevation of HSPs expression in skeletal muscle. The purpose of this study was to determine whether heat stress facilitates the stretch-induced hypertrophy of skeletal muscle cells. Cultured rat myotubes (L6) were plated on collagenized Silastic membranes and incubated at 41 degrees C for 60 and 75 minutes (heat shock). Following the incubation, the cells were subjected two-second stretching and four-second releasing for 4 days at 37 degrees C. Protein concentrations in the homogenates and pellets of the cultured skeletal muscle cells increased under heat shock and/or mechanical stretching. The protein concentration of cells following mechanical stretching following heat shock was significantly higher than that following either heat shock or mechanical stretching alone. HSP72 in supernatants and HSP90 in pellets increased under heat shock and/or mechanical stretching. HSP90 in supernatants decreased following heat shock and/or mechanical stretching. Changes in HSPs and cellular protein concentrations in stressed cells suggest that the expression of HSPs may be closely related with muscular hypertrophy.

Heat stress facilitates stretch-induced hypertrophy of cultured muscle cells. Publishing Authors By Initials

K Yamashita-Goto,Y Ohira,R Okuyama,H Sugiyama,M Honda,T Sugiura,S Yamada,T Akema,T Yoshioka,

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Heat stress facilitates stretch-induced hypertrophy of cultured muscle cells. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of gravitational physiology : a journal of

VOLUME: 9

Page Numbers: P145-6

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ISSN: 1077-9248

DAY: 5

MONTH: Jul

YEAR: 2002

Heat stress facilitates stretch-induced hypertrophy of cultured muscle cells. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Heat stress facilitates stretch-induced hypertrophy of cultured muscle cells.

AFFILIATION: Dept of Physiol, St. Marianna Univ Sch of Med, Kawasaki, Kanagawa, Japan. goto@mbr.sphere.ne.jp

Country: United States

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MEDLINETA: J Gravit Physiol

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