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Heat shock and cold shock in Deinococcus radiodurans.

Heat shock and cold shock in Deinococcus radiodurans. Research Abstract Details 

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  • Heat shock and cold shock in Deinococcus radiodurans. Abstract Text:

    alessandro airoAlessandro Airo,suzanne l chanSuzanne L Chan,zak martinezZak Martinez,manu o plattManu O Platt,jonathan d trentJonathan D Trent,

    On the basis of acquired thermotolerance and cryotolerance, the optimal heat shock and cold shock temperatures have been determined for Deinococcus radiodurans. A heat shock at 42 degrees C maximized survival at the lethal temperature of 52 degrees C and a cold shock at 20 degrees C maximized survival after repeated freeze-thawing. Enhanced survival from heat shock was found to be strongly dependent on growth stage, with its greatest effect shortly after phase. Increased synthesis of a total of 67 proteins during heat shock and 42 proteins during cold shock were observed by two-dimensional polyacrylamide gel electrophoresis (2D PAGE) and autoradiography. Eight of the most highly induced heat shock proteins shown by 2D PAGE were identified by MALDI-MS as Hsp20, GroEL, DnaK, SodA, Csp, Protease I, and two proteins of unknown function.

    Heat shock and cold shock in Deinococcus radiodurans. Publishing Authors By Initials

    a airoA Airo,sl chanSL Chan,z martinezZ Martinez,mo plattMO Platt,jd trentJD Trent,

    For similar abstracts research abstracts see: abstracts research

    PUBMED ID PMID:

    MEDLINE DATE:

    Heat shock and cold shock in Deinococcus radiodurans. Journal Published:

    PUBLICATION TYPE: Research Support, N.I.H., Extr

    Journal: Cell biochemistry and biophysics

    VOLUME: 40

    Page Numbers: 277-88

    Journal Abbreviation: Cell Biochem. Biophys.

    ISSN: 1085-9195

    DAY: 3

    MONTH: 12

    YEAR: 2004

    Heat shock and cold shock in Deinococcus radiodurans. Information

    Number of References:

    LANGUAGE: eng

    NlmUniqueID: 9701934

    Heat shock and cold shock in Deinococcus radiodurans. Keywords Mesh Terms:

    KEYWORDS: Spectrometry, Mass, Matrix-Assisted Lase

    MESH TERMS: metabolism

    Chemical & Substance for Abstract: Heat shock and cold shock in Deinococcus radiodurans. Information

    Substance Name: Heat-Shock Proteins

    Registry Number: 0

    Grant and Affiliation Information for Heat shock and cold shock in Deinococcus radiodurans.

    AFFILIATION: Department of Geological & Environmental Sciences, Stanford University, Stanford, CA, USA.

    Country: United States

    United States Research PublicationUnited States Research Publication

    AGENCY: United States PHS

    GRANT: 103-99ER20354

    ACRONYM:

    MEDLINETA: Cell Biochem Biophys

    REFSOURCE:

    DATABASENAME:

    ACCESSION NUMBER:

    Number Hits: 0

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