Harnessing RNAi for Analyses of Ras Signaling and Transformation.

Harnessing RNAi for Analyses of Ras Signaling and Transformation. Research Abstract Details 

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Harnessing RNAi for Analyses of Ras Signaling and Transformation. Abstract Text:

Angelique W Whitehurst,Michael A White,

The Ras-regulatory network is a loosely defined composition of numerous Ras family members and effector pathways that couple to critical cell-regulatory processes. Investigators are increasingly turning to RNAi-mediated inhibition of gene expression as an effective tool to help generate authentic portraits of Ras protein function in general and to accurately characterize the contribution of Ras family members and Ras effectors to oncogenic transformation in particular. Here we provide detailed protocols for high-efficiency and high-throughput delivery of siRNAs to human cancer cell lines and primary human epithelial cells. In addition, we discuss appropriate controls and limitations for the use of RNAi to derive biologically relevant observations.

Harnessing RNAi for Analyses of Ras Signaling and Transformation. Publishing Authors By Initials

AW Whitehurst,MA White,

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Harnessing RNAi for Analyses of Ras Signaling and Transformation. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Methods in enzymology

VOLUME: 407

Page Numbers: 259-68

Journal Abbreviation: Meth. Enzymol.

ISSN: 0076-6879

DAY: 7

MONTH: 06

YEAR: 2005

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LANGUAGE: eng

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Country: United States

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MEDLINETA: Methods Enzymol

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