Growth-phase dependent differential gene expression in Synechocystis sp. strain PCC 6803 and regulation by a group 2 sigma factor.

Growth-phase dependent differential gene expression in Synechocystis sp. strain PCC 6803 and regulation by a group 2 sigma factor. Research Abstract Details 

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Growth-phase dependent differential gene expression in Synechocystis sp. strain PCC 6803 and regulation by a group 2 sigma factor. Abstract Text:

Jamie S Foster,Abhay K Singh,Lynn J Rothschild,Louis A Sherman,

Cyanobacteria must continually alter their physiological growth state in response to changes in light intensity and their nutritional and physical environment. Under typical laboratory batch growth conditions, cyanobacteria grow exponentially, then transition to a light-limited stage of linear growth before finally reaching a non-growth stationary phase. In this study, we utilized DNA microarrays to profile the expression of genes in the cyanobacterium Synechocystis sp. PCC 6803 to compare exponential and linear growth. We also studied the importance of SigB, a group 2 sigma factor in this cyanobacterium, during the different growth phases. The transcription of approximately 10% of the genes in the wild type were different in the linear, compared to the exponential phase, and our results showed that: (1) many photosynthesis and regulatory genes had lowered transcript levels; (2) individual genes, such as sigH, phrA, and isiA, which encode a group 4 sigma factor, a DNA photolyase, and a Chl-binding protein, respectively, were strongly induced; and, (3) the loss of SigB significantly impacted the differential expression of genes and modulated the changes seen in the wild type in regard to photosynthesis, regulatory and the unknown genes.

Growth-phase dependent differential gene expression in Synechocystis sp. strain PCC 6803 and regulation by a group 2 sigma factor. Publishing Authors By Initials

JS Foster,AK Singh,LJ Rothschild,LA Sherman,

For similar bacteria: cyanobacteria: synechocystis research abstracts see: bacteria: cyanobacteria: synechocystis research

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Growth-phase dependent differential gene expression in Synechocystis sp. strain PCC 6803 and regulation by a group 2 sigma factor. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Archives of microbiology

VOLUME: 187

Page Numbers: 265-79

Journal Abbreviation: Arch. Microbiol.

ISSN: 0302-8933

DAY: 12

MONTH: 12

YEAR: 2006

Growth-phase dependent differential gene expression in Synechocystis sp. strain PCC 6803 and regulation by a group 2 sigma factor. Information

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LANGUAGE: eng

NlmUniqueID: 410427

Growth-phase dependent differential gene expression in Synechocystis sp. strain PCC 6803 and regulation by a group 2 sigma factor. Keywords Mesh Terms:

KEYWORDS: Synechocystis

MESH TERMS: metabolism

Chemical & Substance for Abstract: Growth-phase dependent differential gene expression in Synechocystis sp. strain PCC 6803 and regulation by a group 2 sigma factor. Information

Substance Name: Sigma Factor

Registry Number: 0

Grant and Affiliation Information for Growth-phase dependent differential gene expression in Synechocystis sp. strain PCC 6803 and regulation by a group 2 sigma factor.

AFFILIATION: Department of Microbiology and Cell Science, University of Florida, Space Life Sciences Lab, Kennedy Space Center, FL 32899, USA.

Country: Germany

AGENCY: United States NIAID

GRANT: 5F32AI056967-02

ACRONYM: AI

MEDLINETA: Arch Microbiol

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