Gravity-driven microfluidic particle sorting device with hydrodynamic separation amplification.

Gravity-driven microfluidic particle sorting device with hydrodynamic separation amplification. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Gravity-driven microfluidic particle sorting device with hydrodynamic separation amplification. Abstract Text:

Dongeun Huh,Joong Hwan Bahng,Yibo Ling,Hsien-Hung Wei,Oliver D Kripfgans,J Brian Fowlkes,James B Grotberg,Shuichi Takayama,

This paper describes a simple microfluidic sorting system that can perform size profiling and continuous mass-dependent separation of particles through combined use of gravity (1 g) and hydrodynamic flows capable of rapidly amplifying sedimentation-based separation between particles. Operation of the device relies on two microfluidic transport processes: (i) initial hydrodynamic focusing of particles in a microchannel oriented parallel to gravity and (ii) subsequent sample separation where positional difference between particles with different mass generated by sedimentation is further amplified by hydrodynamic flows whose streamlines gradually widen out due to the geometry of a widening microchannel oriented perpendicular to gravity. The microfluidic sorting device was fabricated in poly(dimethylsiloxane), and hydrodynamic flows in microchannels were driven by gravity without using external pumps. We conducted theoretical and experimental studies on fluid dynamic characteristics of laminar flows in widening microchannels and hydrodynamic amplification of particle separation. Direct trajectory monitoring, collection, and post-analysis of separated particles were performed using polystyrene microbeads with different sizes to demonstrate rapid (<1 min) and high-purity (>99.9%) separation. Finally, we demonstrated biomedical applications of our system by isolating small-sized (diameter <6 microm) perfluorocarbon liquid droplets from polydisperse droplet emulsions, which is crucial in preparing contrast agents for safe, reliable ultrasound medical imaging, tracers for magnetic resonance imaging, or transpulmonary droplets used in ultrasound-based occlusion therapy for cancer treatment. Our method enables straightforward, rapid, real-time size monitoring and continuous separation of particles in simple stand-alone microfabricated devices without the need for bulky and complex external power sources. We believe that this system will provide a useful tool to separate colloids and particles for various analytical and preparative applications and may hold potential for separation of cells or development of diagnostic tools requiring point-of-care sample preparation or testing.

Gravity-driven microfluidic particle sorting device with hydrodynamic separation amplification. Publishing Authors By Initials

D Huh,JH Bahng,Y Ling,HH Wei,OD Kripfgans,JB Fowlkes,JB Grotberg,S Takayama,

For similar water research abstracts see: water research

PUBMED ID PMID:

MEDLINE DATE:

Gravity-driven microfluidic particle sorting device with hydrodynamic separation amplification. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Analytical chemistry

VOLUME: 79

Page Numbers: 1369-76

Journal Abbreviation: Anal. Chem.

ISSN: 0003-2700

DAY: 15

MONTH: Feb

YEAR: 2007

Gravity-driven microfluidic particle sorting device with hydrodynamic separation amplification. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 370536

Gravity-driven microfluidic particle sorting device with hydrodynamic separation amplification. Keywords Mesh Terms:

KEYWORDS: Water

MESH TERMS: chemistry

Chemical & Substance for Abstract: Gravity-driven microfluidic particle sorting device with hydrodynamic separation amplification. Information

Substance Name: Water

Registry Number: 7732-18-5

Grant and Affiliation Information for Gravity-driven microfluidic particle sorting device with hydrodynamic separation amplification.

AFFILIATION: Department of Biomedical Engineering, Department of Radiology, University of Michigan, Ann Arbor, Michigan 48109, USA.

Country: United States

AGENCY: United States NIBIB

GRANT: EB00281

ACRONYM: EB

MEDLINETA: Anal Chem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Gravity-driven microfluidic particle sorting device with hydrodynamic separation amplification Related Publications

• Acoustically detectable cellular-level lung injury induced by fluid mechanical stresses in microfluidic airway systems.
• Application of Lab-on-a-chip technology to drug discovery.
• BAG3 regulates motility and adhesion of epithelial cancer cells.
• Computational replicas: anatomic reconstructions of cerebral vessels as volume numerical grids at three-dimensional angiography.
• Current challenges in non-invasive insulin delivery systems: A comparative review.
• Development of photocrosslinked polyacrylic acid hydrogel as an adhesive for dermatological patches: Involvement of formulation factors in physical properties and pharmacological effects.
• Evaluation of the reliability of nonlinear optimal solutions in pharmaceuticals using a bootstrap resampling technique in combination with Kohonen's self-organizing maps.
• First total synthesis of trimeric indole alkaloid, psychotrimine.
• Gravity-driven microfluidic particle sorting device with hydrodynamic separation amplification.
• Leakage-free bonding of porous membranes into layered microfluidic array systems.
• Measurement of pressure and displacement of the membranous labyrinth in endolymphatic hydrops by the tensile test.
• Overexpression of protease-activated receptors-1,-2, and-4 (PAR-1, -2, and -4) in prostate cancer.
• Probing the unfolding and refolding processes of carbonic anhydrase 2 using electrospray ionization mass spectrometry combined with pH jump.
• Production of antineoplastic agents by plant tissue cultures.
• RNA silencing systems and their relevance to allele-specific DNA methylation in plants.
• Reversible on-demand cell alignment using reconfigurable microtopography.
• Reversible switching of high-speed air-liquid two-phase flows using electrowetting-assisted flow-pattern change.
• Scaling analysis of domain-wall free energy in the Edwards-Anderson Ising spin glass in a magnetic field.
• Simultaneous solution to dark matter and flavor problems of supersymmetry.
• Small volume low mechanical stress cytometry using computer-controlled Braille display microfluidics.
• Spectral intermediates during the reduction of hepatic microsomal cytochrome P-450.
• Splenocytes can replace chimeric cells and maintain allograft tolerance.
• Surgical treatment of hepatocellular carcinoma: Evidence-based outcomes.
• The effect of continuous wavy micropatterns on silicone substrates on the alignment of skeletal muscle myoblasts and myotubes.
• Two Distinct Forms of M-Locus Protein Kinase Localize to the Plasma Membrane and Interact Directly with S-Locus Receptor Kinase to Transduce Self-Incompatibility Signaling in Brassica rapa.
• [A case of successful management of repeated recurrent intrahepatic cholangiocarcinoma by repeated hepatectomy and radiofrequency ablations]
• [Case of Perthes' disease (osteochondritis deformans juvenilis) characterized by myalgia of the entire body.]
• [Neoadjuvant chemotherapy for gastric cancer]
• [Re-evaluation of the visiting system at the pediatric surgery ward]
• [Thrombus formation and its prevention during extracorporeal circulation--effect of preoperative administration with the antiplatelet drugs]