Granulocyte macrophage colony-stimulating factor regulates dendritic cell content of atherosclerotic lesions.

Granulocyte macrophage colony-stimulating factor regulates dendritic cell content of atherosclerotic lesions. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Granulocyte macrophage colony-stimulating factor regulates dendritic cell content of atherosclerotic lesions. Abstract Text:

Zory Shaposhnik,Xuping Wang,Michael Weinstein,Brian J Bennett,Aldons J Lusis,

OBJECTIVE: Recent evidence suggests that dendritic cells may play an important role in atherosclerosis. Based primarily on previous in vitro studies, we hypothesized that granulocyte macrophage colony-stimulating factor (GM-CSF)-deficient mice would have decreased dendritic cells in lesions. METHODS AND RESULTS: To test this, we characterized gene targeted GM-CSF(-/-) mice crossed to hypercholesterolemic low-density lipoprotein receptor null mice. Our results provide conclusive evidence that GM-CSF is a major regulator of dendritic cell formation in vivo. Aortic lesion sections in GM-CSF(-/-) low-density lipoprotein receptor null animals showed a dramatic 60% decrease in the content of dendritic cells as judged by CD11c staining but no change in the overall content of monocyte-derived cells. The GM-CSF-deficient mice exhibited a significant 20% to 50% decrease in the size of aortic lesions, depending on the location of the lesions. Other prominent changes in GM-CSF(-/-) mice were decreased lesional T cell content, decreased autoantibodies to oxidized lipids, and striking disruptions of the elastin fibers adjacent to the lesion. CONCLUSION: Given that GM-CSF is dramatically induced by oxidized lipids in endothelial cells, our data suggest that GM-CSF serves to regulate dendritic cell formation in lesions and that this, in turn, influences inflammation, plaque growth and possibly plaque stability.

Granulocyte macrophage colony-stimulating factor regulates dendritic cell content of atherosclerotic lesions. Publishing Authors By Initials

Z Shaposhnik,X Wang,M Weinstein,BJ Bennett,AJ Lusis,

For similar investigative techniques: epidemiologic methods: statistics as topic: sensitivity and specificity research abstracts see: investigative techniques: epidemiologic methods: statistics as topic: sensitivity and specificity research

PUBMED ID PMID:

MEDLINE DATE:

Granulocyte macrophage colony-stimulating factor regulates dendritic cell content of atherosclerotic lesions. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Arteriosclerosis, thrombosis, and vascular biology

VOLUME: 27

Page Numbers: 621-7

Journal Abbreviation: Arterioscler. Thromb. Vasc. Bi

ISSN: 1524-4636

DAY: 7

MONTH: 12

YEAR: 2006

Granulocyte macrophage colony-stimulating factor regulates dendritic cell content of atherosclerotic lesions. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9505803

Granulocyte macrophage colony-stimulating factor regulates dendritic cell content of atherosclerotic lesions. Keywords Mesh Terms:

KEYWORDS: Sensitivity and Specificity

MESH TERMS: physiology

Chemical & Substance for Abstract: Granulocyte macrophage colony-stimulating factor regulates dendritic cell content of atherosclerotic lesions. Information

Substance Name: Granulocyte-Macrophage Colony-Stimulatin

Registry Number: 83869-56-1

Grant and Affiliation Information for Granulocyte macrophage colony-stimulating factor regulates dendritic cell content of atherosclerotic lesions.

AFFILIATION: Department of Molecular and Medical Pharmacology, UCLA School of Medicine, Los Angeles, CA 90095, USA.

Country: United States

AGENCY: United States NHLBI

GRANT: P01 HL-30568

ACRONYM: HL

MEDLINETA: Arterioscler Thromb Vasc Biol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Granulocyte macrophage colony-stimulating factor regulates dendritic cell content of atherosclerotic lesions Related Publications

• A genome-wide set of congenic mouse strains derived from CAST/Ei on a C57BL/6 background.
• Apolipoprotein A-II: a protein in search of a function.
• Apolipoprotein AII Is a Regulator of Very Low Density Lipoprotein Metabolism and Insulin Resistance.
• Association of stearoyl-CoA desaturase 1 activity with familial combined hyperlipidemia.
• Granulocyte macrophage colony-stimulating factor regulates dendritic cell content of atherosclerotic lesions.
• High-resolution mapping of gene expression using association in an outbred mouse stock.
• Identification of Abcc6 as the major causal gene for dystrophic cardiac calcification in mice through integrative genomics.
• Identification of pathways for atherosclerosis in mice: integration of quantitative trait locus analysis and global gene expression data.
• Integrating genetic and gene expression data: application to cardiovascular and metabolic traits in mice.
• Integrating global gene expression analysis and genetics.
• Linkage between the APOB gene and serum ApoB levels in a large pedigree from the Bogalusa Heart Study.
• Localization of the mouse glucocorticoid receptor-interacting protein 1 gene (Grip1) to proximal chromosome 1 by linkage analysis.
• Mapping, genetic isolation, and characterization of genetic loci that determine resistance to atherosclerosis in C3H mice.
• Metabolic syndrome as a modifier of atherosclerosis in murine models.
• Network-centered view of coronary artery disease.
• Trypanosoma congolense: paraoxonase 1 prolongs survival of infected mice.