Grafting of material-binding function into antibodies Functionalization by peptide grafting.

Grafting of material-binding function into antibodies Functionalization by peptide grafting. Research Abstract Details 

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Grafting of material-binding function into antibodies Functionalization by peptide grafting. Abstract Text:

Takamitsu Hattori,Mitsuo Umetsu,Takeshi Nakanishi,Kouhei Tsumoto,Satoshi Ohara,Hiroya Abe,Makio Naito,Ryutaro Asano,Tadafumi Adschiri,Izumi Kumagai,Takamitsu Hattori,Mitsuo Umetsu,Takeshi Nakanishi,Kouhei Tsumoto,Satoshi Ohara,Hiroya Abe,Makio Naito,Ryutaro Asano,Tadafumi Adschiri,Izumi Kumagai,

Quite recently, a few antibodies against bulk material surface have been selected from a human repertoire antibody library, and they are attracting immense interest in the bottom-up integration of nanomaterials. Here, we constructed antibody fragments with binding affinity and specificity for nonbiological inorganic material surfaces by grafting material-binding peptides into loops of the complementarity determining region (CDR) of antibodies. Loops were replaced by peptides with affinity for zinc oxide and silver material surfaces. Selection of CDR loop for replacement was critical to the functionalization of the grafted fragments; the grafting of material-binding peptide into the CDR2 loop functionalized the antibody fragments with the same affinity and selectivity as the peptides used. Structural insight on the scaffold fragment used implies that material-binding peptide should be grafted onto the most exposed CDR loop on scaffold fragment. We show that the CDR-grafting technique leads to a build-up creation of the antibody with affinity for nonbiological materials.

Grafting of material-binding function into antibodies Functionalization by peptide grafting. Publishing Authors By Initials

T Hattori,M Umetsu,T Nakanishi,K Tsumoto,S Ohara,H Abe,M Naito,R Asano,T Adschiri,I Kumagai,T Hattori,M Umetsu,T Nakanishi,K Tsumoto,S Ohara,H Abe,M Naito,R Asano,T Adschiri,I Kumagai,

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Grafting of material-binding function into antibodies Functionalization by peptide grafting. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Biochemical and biophysical research communication

VOLUME: 365

Page Numbers: 751-7

Journal Abbreviation: Biochem. Biophys. Res. Commun.

ISSN: 1090-2104

DAY: 26

MONTH: 11

YEAR: 2007

Grafting of material-binding function into antibodies Functionalization by peptide grafting. Information

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LANGUAGE: eng

NlmUniqueID: 372516

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Grant and Affiliation Information for Grafting of material-binding function into antibodies Functionalization by peptide grafting.

AFFILIATION: Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University, Aoba 6-6-11-606, Aramaki, Aoba-ku, Sendai 980-8579, Japan.

Country: United States

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MEDLINETA: Biochem Biophys Res Commun

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