Glypican-1 regulates anaphase promoting complex/cyclosome substrates and cell cycle progression in endothelial cells.

Glypican-1 regulates anaphase promoting complex/cyclosome substrates and cell cycle progression in endothelial cells. Research Abstract Details 

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Glypican-1 regulates anaphase promoting complex/cyclosome substrates and cell cycle progression in endothelial cells. Abstract Text:

Glypican-1 (GPC1), a member of the mammalian glypican family of heparan sulfate proteoglycans, is highly expressed in glioma blood vessel endothelial cells (ECs). In this study, we investigated the role of GPC1 in EC replication by manipulating GPC1 expression in cultured mouse brain ECs. Moderate GPC1 overexpression stimulates EC growth, but proliferation is significantly suppressed when GPC1 expression is either knocked down or the molecule is highly overexpressed. Flow cytometric and biochemical analyses show that high or low expression of GPC1 causes cell cycle arrest at mitosis or the G2 phase of the cell cycle, accompanied by endoreduplication and consequently polyploidization. We further show that GPC1 inhibits the anaphase-promoting complex/cyclosome (APC/C)-mediated degradation of mitotic cyclins and securin. High levels of GPC1 induce metaphase arrest and centrosome overproduction, alterations that are mimicked by overexpression of cyclin B1 and cyclin A, respectively. These observations suggest that GPC1 regulates EC cell cycle progression at least partially by modulating APC/C-mediated degradation of mitotic cyclins and securin.

Glypican-1 regulates anaphase promoting complex/cyclosome substrates and cell cycle progression in endothelial cells. Publishing Authors By Initials

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Glypican-1 regulates anaphase promoting complex/cyclosome substrates and cell cycle progression in endothelial cells. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Molecular biology of the cell

VOLUME: 19

Page Numbers: 2789-801

Journal Abbreviation: Mol. Biol. Cell

ISSN: 1059-1524

DAY: 16

MONTH: 04

YEAR: 2008

Glypican-1 regulates anaphase promoting complex/cyclosome substrates and cell cycle progression in endothelial cells. Information

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LANGUAGE: eng

NlmUniqueID: 9201390

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Grant and Affiliation Information for Glypican-1 regulates anaphase promoting complex/cyclosome substrates and cell cycle progression in endothelial cells.

AFFILIATION: *Department of Pathology and Laboratory Medicine, University of Wisconsin, Madison, WI 53792.

Country: United States

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MEDLINETA: Mol Biol Cell

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