Glycosaminoglycans and glycoproteins isolated from rabbit femur.

Glycosaminoglycans and glycoproteins isolated from rabbit femur. Research Abstract Details 

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Glycosaminoglycans and glycoproteins isolated from rabbit femur. Abstract Text:

M Masubuchi,S Miura,Z Yosizawa,

1. Complex carbohydrate fractions were extracted successively with 40% aqueous EDTA (pH 7.4) and 6M urea (PH 7.8) FROM ACETONE-DRIED bone powder of rabbit femur. 2. The carbohydrate fraction extracted with EDTA (E=Fr) was separated into five fractions,D1approximatelyD5 by DEAE-Dephadex A-50 column chromatography. Chemical and infrared spectral analyses, and enzymatic digestion indicate that D2 contained lessacidic glycoprotein, D3 contained sialoglycoprotein, D4 contained a low sulfated proteokeratan sulfate-like substance, and d5 contained glycoprotein-bound chondroitin sulfate A plus protein-free chondroitin sulfate A. 3. Two fractions, HU-D1 and HU-D2, were isolated from the carbohydrate fraction extracted with urea (HU-Fr) by successive digestion with collagenase [EC 3.4.99.5] and pronase, followed by gel-filtration on Sephadex G-100 and then DEAE-Sephadex A-50 column chromatography. HU-D1 and HU-D2 contained a low sulfated keratan sulfate-like substance linked to peptide and glycopeptide-bound chondroitin sulfated keratan sulfate-like substance linked to peptide and glycopeptide-bound chondroitin sulfate A, respectively. 4. The present findings indicate that rabbit femur contains low sulfated proteokeratan sulfate-like substances with varying sulfate contents and glycoprotein-bound chondroitin sulfate A as the principal glycosaminoglycans. The macromolecules bound more tightly to the tissue contain much more sulfate than the corresponding loosely bound ones.

Glycosaminoglycans and glycoproteins isolated from rabbit femur. Publishing Authors By Initials

M Masubuchi,S Miura,Z Yosizawa,

For similar organic chemicals: urea research abstracts see: organic chemicals: urea research

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Glycosaminoglycans and glycoproteins isolated from rabbit femur. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 77

Page Numbers: 617-26

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Mar

YEAR: 1975

Glycosaminoglycans and glycoproteins isolated from rabbit femur. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Glycosaminoglycans and glycoproteins isolated from rabbit femur. Keywords Mesh Terms:

KEYWORDS: Urea

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Glycosaminoglycans and glycoproteins isolated from rabbit femur. Information

Substance Name: Microbial Collagenase

Registry Number: EC 3.4.24.3

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Country: JAPAN

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MEDLINETA: J Biochem

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