Glycolipid Studies in Small Intestine and Pancreas of alpha1,3-Galactosyltransferase Knockout Miniature Swine: alpha1,3GALT-KO Animals Lack alphaGAL Antigens and Contain Novel Blood Group H Compounds.

Glycolipid Studies in Small Intestine and Pancreas of alpha1,3-Galactosyltransferase Knockout Miniature Swine: alpha1,3GALT-KO Animals Lack alphaGAL Antigens and Contain Novel Blood Group H Compounds. Research Abstract Details 

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Glycolipid Studies in Small Intestine and Pancreas of alpha1,3-Galactosyltransferase Knockout Miniature Swine: alpha1,3GALT-KO Animals Lack alphaGAL Antigens and Contain Novel Blood Group H Compounds. Abstract Text:

M Diswall,J ,H-J Schuurman,F J M F Dor,L Rydberg,M E Breimer,

BACKGROUND: To avoid hyperacute rejection of xeno-organs, alpha1,3-galactosyltransferase knock-out (GalT-KO) pigs have been produced. However, Galalpha1,3Gal (Gal) determinant elimination may expose cryptic carbohydrate antigens and/or generate new antigens that might interfere with the human immune response. METHODS: Glycolipids isolated from small intestine and pancreas of two GalT-KO and one wild-type (WT) pig were tested for immune reactivity with antibodies on thin-layer chromatograms after separation by high-performance liquid chromatography, and selected fractions were analysed by proton NMR spectroscopy. RESULTS: Immunostaining using purified human anti-Gal Abs revealed that tissues from WT animals express large amounts of Gal-antigens whereas GalT-KO tissues lacked these antigens. Proton NMR spectroscopy on small intestine fractions revealed both linear and branched nona- and decaglycosylceramides, respectively, with terminal Gal-epitopes. In corresponding GalT-KO fractions, Gal-epitopes seemed to be replaced by terminal alpha1,2fucoses. Two novel branched blood group H compounds was found in the GalT-KO intestine. CONCLUSIONS: The structural complexity of alphaGal-terminating antigens in the WT organs is very high. Knockout of alpha1,3GalT by gene-targeting results in elimination of Gal-determinants. In addition structurally novel alpha1,2fucose-terminated blood group H compounds were identified in the GalT-KO tissue. These compounds are not expected to be recognized by the human immune system.

Glycolipid Studies in Small Intestine and Pancreas of alpha1,3-Galactosyltransferase Knockout Miniature Swine: alpha1,3GALT-KO Animals Lack alphaGAL Antigens and Contain Novel Blood Group H Compounds. Publishing Authors By Initials

M Diswall,J ,HJ Schuurman,FJ Dor,L Rydberg,ME Breimer,

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Glycolipid Studies in Small Intestine and Pancreas of alpha1,3-Galactosyltransferase Knockout Miniature Swine: alpha1,3GALT-KO Animals Lack alphaGAL Antigens and Contain Novel Blood Group H Compounds. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Transplantation proceedings

VOLUME: 40

Page Numbers: 543-6

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ISSN: 0041-1345

DAY: 31

MONTH: Mar

YEAR: 2008

Glycolipid Studies in Small Intestine and Pancreas of alpha1,3-Galactosyltransferase Knockout Miniature Swine: alpha1,3GALT-KO Animals Lack alphaGAL Antigens and Contain Novel Blood Group H Compounds. Information

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LANGUAGE: eng

NlmUniqueID: 243532

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