Glutamine synthetase in preparations of RNA polymerase of Escherichia coli: a novel purification procedure.

Glutamine synthetase in preparations of RNA polymerase of Escherichia coli: a novel purification procedure. Research Abstract Details 

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Glutamine synthetase in preparations of RNA polymerase of Escherichia coli: a novel purification procedure. Abstract Text:

T Takahashi,Y Adachi,

A novel procedure of operational ease and reproducibility for the partial purification of DNA-dependent RNA polymerase [EC 2.7.7.6] from Escherichia coli is reported. It utilizes liquid phase partitions with polyethylene glycol and Dextran, ammonium sulfate fractionations and chromatography on a QAE-Sephadex A-50 column. A copurified protein in the partially purified preparation was isolated and identifed as glutamine synthetase [L-glutamate : ammonia ligase (ADP) EC 6.3.1.2].

Glutamine synthetase in preparations of RNA polymerase of Escherichia coli: a novel purification procedure. Publishing Authors By Initials

T Takahashi,Y Adachi,

For similar enzymes and coenzymes: enzymes: ligases: carbon-nitrogen ligases: amide synthases: glutamate-ammonia ligase research abstracts see: enzymes and coenzymes: enzymes: ligases: carbon-nitrogen ligases: amide synthases: glutamate-ammonia ligase research

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Glutamine synthetase in preparations of RNA polymerase of Escherichia coli: a novel purification procedure. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 91

Page Numbers: 1719-24

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: May

YEAR: 1982

Glutamine synthetase in preparations of RNA polymerase of Escherichia coli: a novel purification procedure. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Glutamine synthetase in preparations of RNA polymerase of Escherichia coli: a novel purification procedure. Keywords Mesh Terms:

KEYWORDS: Glutamate-Ammonia Ligase

MESH TERMS: isolation & purification

Chemical & Substance for Abstract: Glutamine synthetase in preparations of RNA polymerase of Escherichia coli: a novel purification procedure. Information

Substance Name: Glutamate-Ammonia Ligase

Registry Number: EC 6.3.1.2

Grant and Affiliation Information for Glutamine synthetase in preparations of RNA polymerase of Escherichia coli: a novel purification procedure.

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Country: JAPAN

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MEDLINETA: J Biochem

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