Glutamate-induced exocytosis of glutamate from astrocytes.

Glutamate-induced exocytosis of glutamate from astrocytes. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Glutamate-induced exocytosis of glutamate from astrocytes. Abstract Text:

Jun Xu,Hong Peng,Ning Kang,Zhuo Zhao,Jane H-C Lin,Patric K Stanton,Jian Kang,

Recent studies indicate that astrocytes can play a much more active role in neuronal circuits than previously believed, by releasing neurotransmitters such as glutamate and ATP. Here we report that local application of glutamate or glutamine synthetase inhibitors induces astrocytic release of glutamate, which activates a slowly decaying transient inward current (SIC) in CA1 pyramidal neurons and a transient inward current in astrocytes in hippocampal slices. The occurrence of SICs was accompanied by an appearance of large vesicles around the puffing pipette. The frequency of SICs was positively correlated with [glutamate]o. EM imaging of anti-glial fibrillary acid protein-labeled astrocytes showed glutamate-induced large astrocytic vesicles. Imaging of FM 1-43 fluorescence using two-photon laser scanning microscopy detected glutamate-induced formation and fusion of large vesicles identified as FM 1-43-negative structures. Fusion of large vesicles, monitored by collapse of vesicles with a high intensity FM 1-43 stain in the vesicular membrane, coincided with SICs. Glutamate induced two types of large vesicles with high and low intravesicular [Ca2+]. The high [Ca2+] vesicle plays a major role in astrocytic release of glutamate. Vesicular fusion was blocked by infusing the Ca2+ chelator, 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid, or the SNARE blocker, tetanus toxin, suggesting Ca2+- and SNARE-dependent fusion. Infusion of the vesicular glutamate transport inhibitor, Rose Bengal, reduced astrocytic glutamate release, suggesting the involvement of vesicular glutamate transports in vesicular transport of glutamate. Our results demonstrate that local [glutamate]o increases induce formation and exocytotic fusion of glutamate-containing large astrocytic vesicles. These large vesicles could play important roles in the feedback control of neuronal circuits and epileptic seizures.

Glutamate-induced exocytosis of glutamate from astrocytes. Publishing Authors By Initials

J Xu,H Peng,N Kang,Z Zhao,JH Lin,PK Stanton,J Kang,

For similar proteins: membrane proteins: membrane fusion proteins: snare proteins research abstracts see: proteins: membrane proteins: membrane fusion proteins: snare proteins research

PUBMED ID PMID:

MEDLINE DATE:

Glutamate-induced exocytosis of glutamate from astrocytes. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: The Journal of biological chemistry

VOLUME: 282

Page Numbers: 24185-97

Journal Abbreviation: J. Biol. Chem.

ISSN: 0021-9258

DAY: 21

MONTH: 06

YEAR: 2007

Glutamate-induced exocytosis of glutamate from astrocytes. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985121

Glutamate-induced exocytosis of glutamate from astrocytes. Keywords Mesh Terms:

KEYWORDS: SNARE Proteins

MESH TERMS: physiology

Chemical & Substance for Abstract: Glutamate-induced exocytosis of glutamate from astrocytes. Information

Substance Name: Calcium

Registry Number: 7440-70-2

Grant and Affiliation Information for Glutamate-induced exocytosis of glutamate from astrocytes.

AFFILIATION: Center for Basic Neuroscience, Department of Molecular Genetics, and Howard Hughes Medical Institutes, University of Texas Southwestern Medical Center, Dallas Texas 75390, USA.

Country: United States

AGENCY: United States NINDS

GRANT: NS 39997

ACRONYM: NS

MEDLINETA: J Biol Chem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Glutamate-induced exocytosis of glutamate from astrocytes Related Publications

• Activation of PKC but not of ERK is required for vitamin E-succinate-induced apoptosis of HL-60 cells.
• Attenuation by metallothionein of early cardiac cell death via suppression of mitochondrial oxidative stress results in a prevention of diabetic cardiomyopathy.
• Behavioral models of Parkinson's disease in rodents: a new look at an old problem.
• Breast cancer risk and immune responses in healthy women.
• Calcineurin activation is not necessary for Doxorubicin-induced hypertrophy in H9c2 embryonic rat cardiac cells: involvement of the phosphoinositide 3-kinase-Akt pathway.
• Crystal structures and catalytic mechanism of the Arabidopsis cinnamyl alcohol dehydrogenases AtCAD5 and AtCAD4.
• Crystal structures of vegetative soybean lipoxygenase VLX-B and VLX-D, and comparisons with seed isoforms LOX-1 and LOX-3.
• Evidence of a lysosomal pathway for apoptosis induced by the synthetic retinoid CD437 in human leukemia HL-60 cells.
• Gene transfer of the catabolic inhibitor TIMP-1 increases measured proteoglycans in cells from degenerated human intervertebral discs.
• Melanoma growth is reduced in fat-1 transgenic mice: impact of omega-6/omega-3 essential fatty acids.
• Moyamoya disease with repeated intracranial haemorrhage in two consecutive pregnancies.
• Perceived stress among deaf adults.
• Production of inducible nitric oxide is required for monocytic differentiation of U937 cells induced by vitamin E-succinate.
• Synovial sarcoma of primary bone origin: a rare case in a rare site with atypical features.
• [A case of hemophagocytic syndrome with terminal ileal ulcerations]
• [Analysis and follow up research of 74 cases without psychosis in forensic psychiatric expertise]
• [Bonding behavior of 4f orbital]
• [Comparative study of room-temperature phosphorescence of 1-bromonaphthalene induced by synergetic effect of nonionic surfactants and beta-cyclodextrin]
• [Concept analysis of spiritual care]
• [Construction of urban green space ecosystem by using corridor network: a case study in west urban area of Dongying City, Shandong Province]
• [Correction of pediatric concealed penis by using a modified Devine' s technique]
• [Determination of Levofloxacin by chemiluminescence method]
• [Development of ELISA-kit of quantitative analysis for Zearalenone]
• [Dynamic study of anti-NS5 and ALT in post-transfusion hepatitis C]
• [Effects of constraint-induced movement using self-efficacy enhancing strategies on the upper extremity function of chronic hemiplegic patients]
• [Nicotine dependence, smoking-related attitude, and subjective norms across the stages of change for smoking cessation among adults smokers in a rural area]
• [Pathologic and X-ray features of primary malignant fibrous histiocytoma of bone--a report of 16 cases]
• [Percutaneous nephrolithotripsy with pneumatic and ultrasonic power under B-type ultrasound guidance for treatment of renal calculi in non-dilated collecting system]
• [Study on the silica-induced cytotoxicity and hprt gene mutagenisis in rat lung fibroblasts and alveolar type II epithelial cells]
• [The effects of cognitive behavioral anger management program on self-consciousness and anger in Korean military man]