Glucagon and glucose as major regulators of glycogen metabolism in primary cultured rat hepatocytes.

Glucagon and glucose as major regulators of glycogen metabolism in primary cultured rat hepatocytes. Research Abstract Details 

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Glucagon and glucose as major regulators of glycogen metabolism in primary cultured rat hepatocytes. Abstract Text:

T Nakamura,S Kato,A Ichihara,

The short-term controls of glycogen synthase [EC 2.4.1.11] and glycogen phosphorylase [EC 2.4.1.1] by major regulators, such as insulin, glucose, catecholamine, and glucagon, were compared in a simple, yet organized experimental system, i.e., adult rat hepatocytes in primary culture. Glycogen synthase was activated by glucose markedly and dose-dependently (5-40 mM), but insulin alone (1 X 10(-8) M) activated this enzyme only two-fold. Therefore, activation of the enzyme by the two regulators together was mostly due to activation by glucose. Glucagon at a concentration of 5 X 10(-10) M suppressed this activation almost completely. Glucagon at this concentration activated phosphorylase considerably and this activation was slightly inhibited by insulin. Phenylephrine also activated phosphorylase, and this activation was inhibited by phenoxybenzamine or prazosin, suggesting that activation by catecholamine is through the alpha 1-adrenergic receptor. Similarly a high concentration of glucose diminished the effects of glucagon and phenylephrine. These results suggest that in rat liver, glycogen metabolism is controlled mainly by glucagon, catecholamine, and glucose; the former two activate phosphorylase and inactivate synthase, while glucose activates synthase strongly and inactivates phosphorylase partially. Insulin plays a minor role in both reactions. Thus, the liver is primarily an organ for glucose production, which is regulated by hormones, not for glycogen storage, which is increased only by a high glucose concentration in the portal blood.

Glucagon and glucose as major regulators of glycogen metabolism in primary cultured rat hepatocytes. Publishing Authors By Initials

T Nakamura,S Kato,A Ichihara,

For similar animals: animal population groups: animals, inbred strains: rats, inbred strains research abstracts see: animals: animal population groups: animals, inbred strains: rats, inbred strains research

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Glucagon and glucose as major regulators of glycogen metabolism in primary cultured rat hepatocytes. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 95

Page Numbers: 1691-6

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Jun

YEAR: 1984

Glucagon and glucose as major regulators of glycogen metabolism in primary cultured rat hepatocytes. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Glucagon and glucose as major regulators of glycogen metabolism in primary cultured rat hepatocytes. Keywords Mesh Terms:

KEYWORDS: Rats, Inbred Strains

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Glucagon and glucose as major regulators of glycogen metabolism in primary cultured rat hepatocytes. Information

Substance Name: Glycogen Synthase

Registry Number: EC 2.4.1.11

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Country: JAPAN

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MEDLINETA: J Biochem

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