Globin switches in yolk sac-like primitive and fetal-like definitive red blood cells produced from human embryonic stem cells.

Globin switches in yolk sac-like primitive and fetal-like definitive red blood cells produced from human embryonic stem cells. Research Abstract Details 

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Globin switches in yolk sac-like primitive and fetal-like definitive red blood cells produced from human embryonic stem cells. Abstract Text:

Caihong Qiu,Emmanuel N Olivier,Michelle Velho,Eric E Bouhassira,Caihong Qiu,Emmanuel N Olivier,Michelle Velho,Eric E Bouhassira,

We have previously shown that coculture of human embryonic stem cells (hESCs) for 14 days with immortalized fetal hepatocytes yields CD34(+) cells that can be expanded in serum-free liquid culture into large numbers of megaloblastic nucleated erythroblasts resembling yolk sac-derived cells. We show here that these primitive erythroblasts undergo a switch in hemoglobin (Hb) composition during late terminal erythroid maturation with the basophilic erythroblasts expressing predominantly Hb Gower I (zeta(2)epsilon(2)) and the orthochromatic erythroblasts hemoglobin Gower II (alpha(2)epsilon(2)). This suggests that the switch from Hb Gower I to Hb Gower II, the first hemoglobin switch in humans is a maturation switch not a lineage switch. We also show that extending the coculture of the hESCs with immortalized fetal hepatocytes to 35 days yields CD34(+) cells that differentiate into more developmentally mature, fetal liver-like erythroblasts, that are smaller, express mostly fetal hemoglobin, and can enucleate. We conclude that hESC-derived erythropoiesis closely mimics early human development because the first 2 human hemoglobin switches are recapitulated, and because yolk sac-like and fetal liver-like cells are sequentially produced. Development of a method that yields erythroid cells with an adult phenotype remains necessary, because the most mature cells that can be produced with current systems express less than 2% adult beta-globin mRNA.

Globin switches in yolk sac-like primitive and fetal-like definitive red blood cells produced from human embryonic stem cells. Publishing Authors By Initials

C Qiu,EN Olivier,M Velho,EE Bouhassira,C Qiu,EN Olivier,M Velho,EE Bouhassira,

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Globin switches in yolk sac-like primitive and fetal-like definitive red blood cells produced from human embryonic stem cells. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Blood

VOLUME: 111

Page Numbers: 2400-8

Journal Abbreviation: Blood

ISSN: 0006-4971

DAY: 16

MONTH: 11

YEAR: 2007

Globin switches in yolk sac-like primitive and fetal-like definitive red blood cells produced from human embryonic stem cells. Information

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LANGUAGE: eng

NlmUniqueID: 7603509

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