Global effects of mistranslation from an editing defect in mammalian cells.

Global effects of mistranslation from an editing defect in mammalian cells. Research Abstract Details 

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Global effects of mistranslation from an editing defect in mammalian cells. Abstract Text:

Leslie A Nangle,Candace M Motta,Paul Schimmel,

Aminoacyl-tRNA synthetases prevent mistranslation, or genetic code ambiguity, through specialized editing reactions. Mutations that disrupt editing in bacteria adversely affect cell growth and viability, and recent work in the mouse supports the idea that translational errors caused by an editing defect lead to a neurological disease-like phenotype. To further investigate the connection of mistranslation to cell pathology, we introduced an inducible transgene expressing an editing-deficient valyl-tRNA synthetase into mammalian cells. Introducing mistranslation precipitated a disruption of cell morphology and membrane blebbing, accompanied by activation of caspase-3, consistent with an apoptotic response. Addition of a noncanonical amino acid that is misactivated, but not cleared, by the editing-defective enzyme exacerbated these effects. A special ambiguity-detecting sensor provided direct readout of mistranslation in vivo, supporting the possibility that decreased translational fidelity could be associated with disease.

Global effects of mistranslation from an editing defect in mammalian cells. Publishing Authors By Initials

LA Nangle,CM Motta,P Schimmel,

For similar enzymes and coenzymes: enzymes: ligases: carbon-oxygen ligases: amino acyl-trna synthetases: valine-trna ligase research abstracts see: enzymes and coenzymes: enzymes: ligases: carbon-oxygen ligases: amino acyl-trna synthetases: valine-trna ligase research

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MEDLINE DATE:

Global effects of mistranslation from an editing defect in mammalian cells. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Chemistry & biology

VOLUME: 13

Page Numbers: 1091-100

Journal Abbreviation: Chem. Biol.

ISSN: 1074-5521

DAY: 3

MONTH: Oct

YEAR: 2006

Global effects of mistranslation from an editing defect in mammalian cells. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9500160

Global effects of mistranslation from an editing defect in mammalian cells. Keywords Mesh Terms:

KEYWORDS: Valine-tRNA Ligase

MESH TERMS: metabolism

Chemical & Substance for Abstract: Global effects of mistranslation from an editing defect in mammalian cells. Information

Substance Name: Valine-tRNA Ligase

Registry Number: EC 6.1.1.9

Grant and Affiliation Information for Global effects of mistranslation from an editing defect in mammalian cells.

AFFILIATION: Department of Molecular Biology, The Skaggs Institute for Chemical Biology, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.

Country: England

AGENCY: United States NIGMS

GRANT: GM2356

ACRONYM: GM

MEDLINETA: Chem Biol

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