Global analysis of alternative splicing during T-cell activation.

Global analysis of alternative splicing during T-cell activation. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Global analysis of alternative splicing during T-cell activation. Abstract Text:

Joanna Y Ip,Alan Tong,Qun Pan,Justin D Topp,Benjamin J Blencowe,Kristen W Lynch,

The role of alternative splicing (AS) in eliciting immune responses is poorly understood. We used quantitative AS microarray profiling to survey changes in AS during activation of Jurkat cells, a leukemia-derived T-cell line. Our results indicate that approximately 10-15% of the profiled alternative exons undergo a >10% change in inclusion level during activation. The majority of the genes displaying differential AS levels are distinct from the set of genes displaying differential transcript levels. These two gene sets also have overlapping yet distinct functional roles. For example, genes that show differential AS patterns during T-cell activation are often closely associated with cell-cycle regulation, whereas genes with differential transcript levels are highly enriched in functions associated more directly with immune defense and cytoskeletal architecture. Previously unknown AS events were detected in genes that have important roles in T-cell activation, and these AS level changes were also observed during the activation of normal human peripheral CD4+ and CD8+ lymphocytes. In summary, by using AS microarray profiling, we have discovered many new AS changes associated with T-cell activation. Our results suggest an extensive role for AS in the regulation of the mammalian immune response.

Global analysis of alternative splicing during T-cell activation. Publishing Authors By Initials

JY Ip,A Tong,Q Pan,JD Topp,BJ Blencowe,KW Lynch,

For similar cells: blood cells: leukocytes: leukocytes, mononuclear: lymphocytes: t-lymphocytes research abstracts see: cells: blood cells: leukocytes: leukocytes, mononuclear: lymphocytes: t-lymphocytes research

PUBMED ID PMID:

MEDLINE DATE:

Global analysis of alternative splicing during T-cell activation. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: RNA (New York, N.Y.)

VOLUME: 13

Page Numbers: 563-72

Journal Abbreviation: RNA

ISSN: 1355-8382

DAY: 16

MONTH: 02

YEAR: 2007

Global analysis of alternative splicing during T-cell activation. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9509184

Global analysis of alternative splicing during T-cell activation. Keywords Mesh Terms:

KEYWORDS: T-Lymphocytes

MESH TERMS: immunology

Chemical & Substance for Abstract: Global analysis of alternative splicing during T-cell activation. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Global analysis of alternative splicing during T-cell activation.

AFFILIATION: Banting and Best Department of Medical Research, University of Toronto, Toronto, ONT, Canada.

Country: United States

AGENCY: United States NIGMS

GRANT: R01 GM067719

ACRONYM: GM

MEDLINETA: RNA

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Global analysis of alternative splicing during T-cell activation Related Publications

• Amphibian lipid levels at metamorphosis correlate to post-metamorphic terrestrial survival.
• Brain-derived neurotrophic factor promotes long-term potentiation-related cytoskeletal changes in adult hippocampus.
• Changes in synaptic morphology accompany actin signaling during LTP.
• Chronic hypersensitivity pneumonitis: CT features comparison with pathologic evidence of fibrosis and survival.
• Clinical selection of candidates for mutational testing for cancer susceptibility.
• Current and emerging strategies for the management of sarcoidosis.
• Dietary fish oil deactivates a growth-promoting signaling pathway in hepatoma 7288CTC in Buffalo rats.
• Diindolylquinoxalines: effective indole-based receptors for phosphate anion.
• ENG mutations in MADH4/BMPR1A mutation negative patients with juvenile polyposis.
• Evidence that long-term potentiation occurs within individual hippocampal synapses during learning.
• Familial genes in sporadic disease: common variants of alpha-synuclein gene associate with Parkinson's disease.
• Follicular development during early pregnancy and the estrous cycle of the sow.
• Fyn-mediated phosphorylation of NR2B Tyr-1336 controls calpain-mediated NR2B cleavage in neurons and heterologous systems.
• GAD antibody positive paraneoplastic stiff person syndrome in a patient with renal cell carcinoma.
• Global analysis of alternative splicing during T-cell activation.
• Grade III Gliomas with Imaging Appearances of Glioblastoma should be Managed as Glioblastoma.
• Hereditary cancer syndrome diagnosis: molecular genetic clues and cancer control.
• Large global effective population sizes in Paramecium.
• Nutrient signaling components controlling protein synthesis in striated muscle.
• Paraneoplastic anti-N-methyl-D-aspartate receptor encephalitis associated with ovarian teratoma.
• Pharmacological therapy for Wegener's granulomatosis.
• Presence or absence of lipopolysaccharide O antigens affects type III secretion by Pseudomonas aeruginosa.
• Rapamycin blunts nutrient stimulation of eIF4G, but not PKCepsilon phosphorylation, in skeletal muscle.
• Response to treatment and survival of patients with non-small cell lung cancer undergoing somatic EGFR mutation testing.
• Results from an autonomous underwater vehicle towed hydrophone array experiment in Nantucket Sound.
• Tantalus, a 240MeV Dedicated Source of Synchrotron Radiation, 1968-1986.
• The effect of maternal socio-economic status throughout the lifespan on infant birthweight.
• The relative roles of three DNA repair pathways in preventing Caenorhabditis elegans mutation accumulation.
• Toward a consensus in molecular diagnosis of hereditary nonpolyposis colorectal cancer (Lynch syndrome).
• Who should be sent for genetic testing in hereditary colorectal cancer syndromes?