Germ cell-specific expression of microphthalmia-associated transcription factor mRNA in mouse testis.

Germ cell-specific expression of microphthalmia-associated transcription factor mRNA in mouse testis. Research Abstract Details 

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Germ cell-specific expression of microphthalmia-associated transcription factor mRNA in mouse testis. Abstract Text:

Hideo Saito,Kazuhisa Takeda,Ken-ichi Yasumoto,Haruo Ohtani,Ken-ichi Watanabe,Kazuhiro Takahashi,Atsushi Fukuzaki,Yoichi Arai,Hiroaki Yamamoto,Shigeki Shibahara,

The gene coding for microphthalmia-associated transcription factor (Mitf) contains many promoters that could generate multiple Mitf isoforms with distinct amino-termini, such as ubiquitously expressed Mitf-A and Mitf-H. To gain further insight into Mitf isoform multiplicity and the regulation of the promoter usage of the Mitf gene, we have analyzed the function of the amino-terminal domains of Mitf isoforms and the expression of Mitf mRNA in mouse postnatal testis, which is characterized by spermatogenesis and by a cool temperature because of its unique location. Here we show that the amino-terminal domain of Mitf-A possesses a transactivation activity, as judged by yeast expression analysis. We also show the expression of Mitf-A and Mitf-D mRNAs in testis by PCR-based methods. Moreover, in situ hybridization analysis revealed that an Mitf mRNA, probably representing Mitf-A and/or Mitf-D, is expressed in germ cells, including spermatogonia, spermatocytes that undergo meiosis, and round spermatids with the haploid genome, but is undetectable in elongated spermatids with remodeled and condensed chromatin. Notably, Mitf mRNA is undetectable in somatic Leydig cells and peritubular cells. Therefore, multiple promoters may direct differential expression of the Mitf gene in the testis and contribute to functional diversity of Mitf isoforms.

Germ cell-specific expression of microphthalmia-associated transcription factor mRNA in mouse testis. Publishing Authors By Initials

H Saito,K Takeda,K Yasumoto,H Ohtani,K Watanabe,K Takahashi,A Fukuzaki,Y Arai,H Yamamoto,S Shibahara,

For similar genetic processes: gene expression: transcription, genetic research abstracts see: genetic processes: gene expression: transcription, genetic research

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Germ cell-specific expression of microphthalmia-associated transcription factor mRNA in mouse testis. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 134

Page Numbers: 143-50

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Jul

YEAR: 2003

Germ cell-specific expression of microphthalmia-associated transcription factor mRNA in mouse testis. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Germ cell-specific expression of microphthalmia-associated transcription factor mRNA in mouse testis. Keywords Mesh Terms:

KEYWORDS: Transcription, Genetic

MESH TERMS: genetics

Chemical & Substance for Abstract: Germ cell-specific expression of microphthalmia-associated transcription factor mRNA in mouse testis. Information

Substance Name: Transcription Factors

Registry Number: 0

Grant and Affiliation Information for Germ cell-specific expression of microphthalmia-associated transcription factor mRNA in mouse testis.

AFFILIATION: Department of Molecular Biology and Applied Physiology, Tohoku University School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi 980-8575.

Country: Japan

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MEDLINETA: J Biochem

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