Geranylgeranylacetone ameliorates inflammatory response to lipopolysaccharide (LPS) in murine macrophages: inhibition of LPS binding to the cell surface.

Geranylgeranylacetone ameliorates inflammatory response to lipopolysaccharide (LPS) in murine macrophages: inhibition of LPS binding to the cell surface. Research Abstract Details 

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Geranylgeranylacetone ameliorates inflammatory response to lipopolysaccharide (LPS) in murine macrophages: inhibition of LPS binding to the cell surface. Abstract Text:

Shinsuke Mochida,Tatsuya Matsura,Atsushi Yamashita,Shunsuke Horie,Shuzo Ohata,Chiaki Kusumoto,Tadashi Nishida,Yukari Minami,Yoshimi Inagaki,Yuichi Ishibe,Junya Nakada,Yoshiji Ohta,Kazuo Yamada,

We investigated whether pretreatment with geranylgeranylacetone (GGA), a potent heat shock protein (HSP) inducer, could inhibit proinflammatory cytokine liberation and nitric oxide (NO) production in lipopolysaccharide (LPS)-treated murine macrophages. The levels of NO and tumor necrosis factor-alpha (TNF-alpha) released from murine macrophage RAW 264 cells were increased dose- and time-dependently following treatment with LPS (1 microg/ml). GGA (80 microM) treatment 2 h before LPS addition significantly suppressed TNF-alpha and NO productions at 12 h and 24 h after LPS, respectively, indicating that GGA inhibits activation of macrophages. However, replacement by fresh culture medium before LPS treatment abolished the inhibitory effect of GGA on NO production in LPS-treated cells. Furthermore, GGA inhibited both HSP70 and inducible NO synthase expressions induced by LPS treatment despite an HSP inducer. When it was examined whether GGA interacts with LPS and/or affects expression of Toll-like receptor 4 (TLR4) and CD14 on the cell surface, GGA inhibited the binding of LPS to the cell surface, while GGA did not affect TLR4 and CD14 expressions. These results indicate that GGA suppresses the binding of LPS to the cell surface of macrophages, resulting in inhibiting signal transduction downstream of TLR4.

Geranylgeranylacetone ameliorates inflammatory response to lipopolysaccharide (LPS) in murine macrophages: inhibition of LPS binding to the cell surface. Publishing Authors By Initials

S Mochida,T Matsura,A Yamashita,S Horie,S Ohata,C Kusumoto,T Nishida,Y Minami,Y Inagaki,Y Ishibe,J Nakada,Y Ohta,K Yamada,

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Geranylgeranylacetone ameliorates inflammatory response to lipopolysaccharide (LPS) in murine macrophages: inhibition of LPS binding to the cell surface. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of clinical biochemistry and nutrition

VOLUME: 41

Page Numbers: 115-23

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ISSN: 0912-0009

DAY: 14

MONTH: Sep

YEAR: 2007

Geranylgeranylacetone ameliorates inflammatory response to lipopolysaccharide (LPS) in murine macrophages: inhibition of LPS binding to the cell surface. Information

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LANGUAGE: eng

NlmUniqueID: 8700907

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Grant and Affiliation Information for Geranylgeranylacetone ameliorates inflammatory response to lipopolysaccharide (LPS) in murine macrophages: inhibition of LPS binding to the cell surface.

AFFILIATION: Division of Medical Biochemistry, Department of Pathophysiological and Therapeutic Science, Tottori University Faculty of Medicine, 86 Nishi-cho, Yonago 683-8503, Japan.

Country: Japan

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MEDLINETA: J Clin Biochem Nutr

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